Synthesis and Characterization of Radiogallium-Labeled Cationic Amphiphilic Peptides as Tumor Imaging Agents
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. General Information
2.2. Peptide Synthesis
2.3. Synthesis of Ga-NOTA-CAPs
2.4. Synthesis of 67Ga-NOTA-CAPs
2.5. Preparation of Liposomes
2.6. Measurement of Zeta Potential of the CAP–Liposome Mixtures
2.7. CD Spectroscopy of the CAP–Liposome Mixture
2.8. In Vitro Stability of 67Ga-NOTA-CAPs in Phosphate-Buffered Saline and Mouse Plasma
2.9. Cell Cultures
2.10. Measurement of Zeta Potential of Cell Cultures
2.11. Cellular Uptake Study
2.12. Confocal Fluorescence Microscopic Imaging of Cells Exposed to 67Ga-NOTA-CAPs
2.13. Evaluation of the Internalization Pathway of CAPs
2.14. Tumor Xenograft Model
2.15. Biodistribution of 67Ga-CAPs in Tumor-Bearing Mice
2.16. Statistical Analysis
3. Results
3.1. In Vitro Binding of FITC-Labeled CAPs to Cancer Cells and Healthy Cells
3.2. Zeta Potential and CD Spectra of NOTA-CAPs in the Presence of a Lipid Membrane
3.3. Radiosynthesis and In Vitro Stability of 67Ga-NOTA-CAPs
3.4. In Vitro Cellular Uptake of 67Ga-NOTA-CAPs
3.5. Evaluation of the Internalization Pathway for CAPs
3.6. In Vivo Biodistribution Studies of 67Ga-NOTA-CAPs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fuchigami, T.; Chiga, T.; Yoshida, S.; Oba, M.; Fukushima, Y.; Inoue, H.; Matsuura, A.; Toriba, A.; Nakayama, M. Synthesis and Characterization of Radiogallium-Labeled Cationic Amphiphilic Peptides as Tumor Imaging Agents. Cancers 2021, 13, 2388. https://doi.org/10.3390/cancers13102388
Fuchigami T, Chiga T, Yoshida S, Oba M, Fukushima Y, Inoue H, Matsuura A, Toriba A, Nakayama M. Synthesis and Characterization of Radiogallium-Labeled Cationic Amphiphilic Peptides as Tumor Imaging Agents. Cancers. 2021; 13(10):2388. https://doi.org/10.3390/cancers13102388
Chicago/Turabian StyleFuchigami, Takeshi, Takeshi Chiga, Sakura Yoshida, Makoto Oba, Yu Fukushima, Hiromi Inoue, Akari Matsuura, Akira Toriba, and Morio Nakayama. 2021. "Synthesis and Characterization of Radiogallium-Labeled Cationic Amphiphilic Peptides as Tumor Imaging Agents" Cancers 13, no. 10: 2388. https://doi.org/10.3390/cancers13102388