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Review

Stable Isotope Tracing Metabolomics to Investigate the Metabolic Activity of Bioactive Compounds for Cancer Prevention and Treatment

1
Department of Nutritional Sciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA
2
Department of Pediatrics, Dell Medical School, The University of Texas at Austin, Austin, TX 78723, USA
3
Department of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX 78723, USA
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(8), 2147; https://doi.org/10.3390/cancers12082147
Received: 30 June 2020 / Revised: 29 July 2020 / Accepted: 30 July 2020 / Published: 3 August 2020
(This article belongs to the Special Issue Medicinal Plants and Their Active Ingredients in Cancer)
A major hallmark of cancer is the metabolic reprogramming of cancer cells to fuel tumor growth and proliferation. Various plant-derived bioactive compounds efficiently target the metabolic vulnerabilities of cancer cells and exhibit potential as emerging therapeutic agents. Due to their safety and common use as dietary components, they are also ideal for cancer prevention. However, to render their use as efficient as possible, the mechanism of action of these phytochemicals needs to be well characterized. Stable isotope tracing is an essential technology to study the molecular mechanisms by which nutraceuticals modulate and target cancer metabolism. The use of positionally labeled tracers as exogenous nutrients and the monitoring of their downstream metabolites labeling patterns enable the analysis of the specific metabolic pathway activity, via the relative production and consumption of the labeled metabolites. Although stable isotope tracing metabolomics is a powerful tool to investigate the molecular activity of bioactive compounds as well as to design synergistic nutraceutical combinations, this methodology is still underutilized. This review aims to investigate the research efforts and potentials surrounding the use of stable isotope tracing metabolomics to examine the metabolic alterations mediated by bioactive compounds in cancer. View Full-Text
Keywords: bioactive compounds; natural products; metabolic pathways; stable isotope tracing; cancer prevention; cancer metabolism bioactive compounds; natural products; metabolic pathways; stable isotope tracing; cancer prevention; cancer metabolism
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MDPI and ACS Style

Choudhury, F.K.; Hackman, G.L.; Lodi, A.; Tiziani, S. Stable Isotope Tracing Metabolomics to Investigate the Metabolic Activity of Bioactive Compounds for Cancer Prevention and Treatment. Cancers 2020, 12, 2147. https://doi.org/10.3390/cancers12082147

AMA Style

Choudhury FK, Hackman GL, Lodi A, Tiziani S. Stable Isotope Tracing Metabolomics to Investigate the Metabolic Activity of Bioactive Compounds for Cancer Prevention and Treatment. Cancers. 2020; 12(8):2147. https://doi.org/10.3390/cancers12082147

Chicago/Turabian Style

Choudhury, Feroza K., G. L. Hackman, Alessia Lodi, and Stefano Tiziani. 2020. "Stable Isotope Tracing Metabolomics to Investigate the Metabolic Activity of Bioactive Compounds for Cancer Prevention and Treatment" Cancers 12, no. 8: 2147. https://doi.org/10.3390/cancers12082147

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