Index of Cancer-Associated Fibroblasts Is Superior to the Epithelial–Mesenchymal Transition Score in Prognosis Prediction
Abstract
:1. Introduction
2. Results
2.1. The Oral Cancer Cell Line with a Higher EMT Score Is Associated with Stronger Stromal Content In Vivo
2.2. OC3 Tumor Cells Displayed a Strong Innate Immune Response in Xenograft Tissues
2.3. Upregulated Expression of Extracellular Matrix (ECM) and Fibroblast-Related Genes in OC3 CDXs
2.4. The Clinical Relevance of Fn1 and TGFβ Axis Genes in Clinical OSCC Datasets
2.5. The Index of Cancer-Associated Fibroblasts Is Better than the EMT Score in Overall Survival Prediction for Cancer Patients
2.6. Comparison of CAF Index and EMT Score Composed of 76-Gene Signature (76GS)
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Antibodies
4.3. Immunohistochemical Staining
4.4. Animal Experiment
4.5. mRNA-Seq Analysis
4.6. Bioinformatics Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Availability of Data and Materials
Appendix A
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Ko, Y.-C.; Lai, T.-Y.; Hsu, S.-C.; Wang, F.-H.; Su, S.-Y.; Chen, Y.-L.; Tsai, M.-L.; Wu, C.-C.; Hsiao, J.-R.; Chang, J.-Y.; et al. Index of Cancer-Associated Fibroblasts Is Superior to the Epithelial–Mesenchymal Transition Score in Prognosis Prediction. Cancers 2020, 12, 1718. https://doi.org/10.3390/cancers12071718
Ko Y-C, Lai T-Y, Hsu S-C, Wang F-H, Su S-Y, Chen Y-L, Tsai M-L, Wu C-C, Hsiao J-R, Chang J-Y, et al. Index of Cancer-Associated Fibroblasts Is Superior to the Epithelial–Mesenchymal Transition Score in Prognosis Prediction. Cancers. 2020; 12(7):1718. https://doi.org/10.3390/cancers12071718
Chicago/Turabian StyleKo, Ying-Chieh, Ting-Yu Lai, Shu-Ching Hsu, Fu-Hui Wang, Sheng-Yao Su, Yu-Lian Chen, Min-Lung Tsai, Chung-Chun Wu, Jenn-Ren Hsiao, Jang-Yang Chang, and et al. 2020. "Index of Cancer-Associated Fibroblasts Is Superior to the Epithelial–Mesenchymal Transition Score in Prognosis Prediction" Cancers 12, no. 7: 1718. https://doi.org/10.3390/cancers12071718