Neuroprotection of Radiosensitive Juvenile Mice by Ultra-High Dose Rate FLASH Irradiation
Abstract
:1. Introduction
2. Results
2.1. FLASH-RT Preserves Memory Updating in OUL over Time
2.2. FLASH-RT Minimizes Radiation-Induced Novel Object Memory Impairments as Well as Anxiety-Like Behaviors
2.3. FLASH-RT Spares the Neurogenic Niche in Juvenile Mice
2.4. FLASH-RT Preserves Neurogenesis in Juvenile Mice
2.5. Attenuation of Activated Microglia by FLASH-RT
2.6. Preservation of the Endocrine System by FLASH-RT
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Whole Brain Irradiations
4.3. Cognitive Testing
4.4. Apparatus
4.5. Objects in Updated Locations (OUL) Task
4.6. Novel Object Recognition (NOR)
4.7. Light-Dark Box (LDB) Test
4.8. Social Interaction Test (SIT)
4.9. Fear Extinction Testing
4.10. BrdU Treatment and Immunohistochemistry Analyses
4.11. Plasma Growth Hormone Quantification
4.12. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Delivery Mode | Prescribed Dose (Gy) | Graphite Applicator Type and Size (mm) | Beam Parameters | ||||||
---|---|---|---|---|---|---|---|---|---|
Source-to-Surface Distance (mm) | Pulse Repetition Frequency (Hz) | Pulse Width (µs) | Number of Pulses | Treatment Time (s) | Mean Dose Rate (Gy/s) | InstantanEous Dose Rate (Gy/s) | |||
CONV | 8 | Semicircular ∅17 | 798 | 10 | 1.0 | 1033 | 103.2 | 0.077 | 7.7 × 103 |
FLASH | 8 | Semicircular ∅17 | 383 | 100 | 1.8 | 1 | 1.8 × 10−6 | 4.4 × 106 | 4.4 × 106 |
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Alaghband, Y.; Cheeks, S.N.; Allen, B.D.; Montay-Gruel, P.; Doan, N.-L.; Petit, B.; Jorge, P.G.; Giedzinski, E.; Acharya, M.M.; Vozenin, M.-C.; et al. Neuroprotection of Radiosensitive Juvenile Mice by Ultra-High Dose Rate FLASH Irradiation. Cancers 2020, 12, 1671. https://doi.org/10.3390/cancers12061671
Alaghband Y, Cheeks SN, Allen BD, Montay-Gruel P, Doan N-L, Petit B, Jorge PG, Giedzinski E, Acharya MM, Vozenin M-C, et al. Neuroprotection of Radiosensitive Juvenile Mice by Ultra-High Dose Rate FLASH Irradiation. Cancers. 2020; 12(6):1671. https://doi.org/10.3390/cancers12061671
Chicago/Turabian StyleAlaghband, Yasaman, Samantha N. Cheeks, Barrett D. Allen, Pierre Montay-Gruel, Ngoc-Lien Doan, Benoit Petit, Patrik Goncalves Jorge, Erich Giedzinski, Munjal M. Acharya, Marie-Catherine Vozenin, and et al. 2020. "Neuroprotection of Radiosensitive Juvenile Mice by Ultra-High Dose Rate FLASH Irradiation" Cancers 12, no. 6: 1671. https://doi.org/10.3390/cancers12061671