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Cancers
Volume 12
Issue 6
10.3390/cancers12061589
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Article

Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy

by
Theresa Suckert
1,2,*,
Treewut Rassamegevanon
1,2,
Johannes Müller
2,3,
Antje Dietrich
1,2,
Antonia Graja
1,2,
Michael Reiche
2,4,
Steffen Löck
1,2,5,
Mechthild Krause
1,2,3,4,5,
Elke Beyreuther
2,6 and
Cläre von Neubeck
1,2,7
1
German Cancer Consortium (DKTK), Partner Site Dresden and German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
2
OncoRay—National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Helmholtz-Zentrum Dresden—Rossendorf, 01309 Dresden, Germany
3
Institute of Radiooncology—OncoRay, Helmholtz-Zentrum Dresden—Rossendorf, 01328 Dresden, Germany
4
National Center for Tumor Diseases (NCT), Partner Site Dresden, 01307 Dresden, Germany
5
Department of Radiotherapy and Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, 01309 Dresden, Germany
6
Helmholtz-Zentrum Dresden—Rossendorf, Institute of Radiation Physics, 01328 Dresden, Germany
7
Department of Particle Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(6), 1589; https://doi.org/10.3390/cancers12061589
Received: 30 March 2020 / Revised: 16 May 2020 / Accepted: 9 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Patient-Derived Xenograft-Models in Cancer Research)
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Abstract

A challenge in cancer research is the definition of reproducible, reliable, and practical models, which reflect the effects of complex treatment modalities and the heterogeneous response of patients. Proton beam radiotherapy (PBRT), relative to conventional photon-based radiotherapy, offers the potential for iso-effective tumor control, while protecting the normal tissue surrounding the tumor. However, the effects of PBRT on the tumor microenvironment and the interplay with newly developed chemo- and immunotherapeutic approaches are still open for investigation. This work evaluated thin-cut tumor slice cultures (TSC) of head and neck cancer and organotypic brain slice cultures (OBSC) of adult mice brain, regarding their relevance for translational radiooncology research. TSC and OBSC were treated with PBRT and investigated for cell survival with a lactate dehydrogenase (LDH) assay, DNA repair via the DNA double strand break marker γH2AX, as well as histology with regards to morphology. Adult OBSC failed to be an appropriate model for radiobiological research questions. However, histological analysis of TSC showed DNA damage and tumor morphological results, comparable to known in vivo and in vitro data, making them a promising model to study novel treatment approaches in patient-derived xenografts or primary tumor material. View Full-Text
Keywords: tumor biology; thin-cut tissue slices; proton beam radiotherapy; head and neck cancer; organotypic brain slice culture; DNA damage tumor biology; thin-cut tissue slices; proton beam radiotherapy; head and neck cancer; organotypic brain slice culture; DNA damage
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Suckert, T.; Rassamegevanon, T.; Müller, J.; Dietrich, A.; Graja, A.; Reiche, M.; Löck, S.; Krause, M.; Beyreuther, E.; von Neubeck, C. Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy. Cancers 2020, 12, 1589. https://doi.org/10.3390/cancers12061589

AMA Style

Suckert T, Rassamegevanon T, Müller J, Dietrich A, Graja A, Reiche M, Löck S, Krause M, Beyreuther E, von Neubeck C. Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy. Cancers. 2020; 12(6):1589. https://doi.org/10.3390/cancers12061589

Chicago/Turabian Style

Suckert, Theresa, Treewut Rassamegevanon, Johannes Müller, Antje Dietrich, Antonia Graja, Michael Reiche, Steffen Löck, Mechthild Krause, Elke Beyreuther, and Cläre von Neubeck. 2020. "Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy" Cancers 12, no. 6: 1589. https://doi.org/10.3390/cancers12061589

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