Next Article in Journal
CITK Loss Inhibits Growth of Group 3 and Group 4 Medulloblastoma Cells and Sensitizes Them to DNA-Damaging Agents
Previous Article in Journal
Moving the Target on the Optimal Adjuvant Strategy for Resected Pancreatic Cancers: A Systematic Review with Meta-Analysis
Open AccessArticle

Molecular Profiling of Keratinocyte Skin Tumors Links Staphylococcus aureus Overabundance and Increased Human β-Defensin-2 Expression to Growth Promotion of Squamous Cell Carcinoma

1
Institute of Pathology, Medical University of Graz, Neue Stiftingtalstrasse 6, 8010 Graz, Austria
2
Theodor Escherich Laboratory for Medical Microbiome Research, Medical University of Graz, Auenbruggerplatz 15, 8036 Graz, Austria
3
Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036 Graz, Austria
4
BioTechMed, Interuniversity Cooperation, Mozartgasse 12/11, 8010 Graz, Austria
5
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany
6
Department of Dermatology, Medical University of Graz, Auenbruggerplatz 8, 8036 Graz, Austria
7
Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Düsseldorf, Germany
8
Department of Translational Skin Cancer Research and Department of Dermatology, German Cancer Consortium (DKTK), University Hospital Essen, Universitätsstrasse 1, 45141 Essen, Germany
*
Authors to whom correspondence should be addressed.
Cancers 2020, 12(3), 541; https://doi.org/10.3390/cancers12030541
Received: 8 November 2019 / Revised: 12 February 2020 / Accepted: 21 February 2020 / Published: 26 February 2020
The skin microbiota plays a prominent role in health and disease; however, its contribution to skin tumorigenesis is not well understood. We comparatively assessed the microbial community compositions from excision specimens of the main human non-melanoma skin cancers, actinic keratosis (AK), squamous cell carcinoma (SCC) and basal cell carcinoma (BCC). Keratinocyte skin tumors are characterized by significantly different microbial community compositions, wherein AK and SCC are more similar to each other than to BCC. Notably, in SCC, which represents the advanced tumor entity and frequently develops from AK, overabundance of Staphylococcus aureus, a known skin pathogen, was noted. Moreover, S. aureus overabundance was significantly associated with increased human β-defensin-2 (hBD-2) expression in SCC. By challenging human SCC cell lines with S. aureus, a specific induction of hBD-2 expression and increased tumor cell growth was seen. Increased proliferation was also induced by directly challenging SCC cells with hBD-2. Together, our data indicate that a changed microbial community composition in SCC, specified by S. aureus overabundance, might promote tumor cell growth via modulation of hBD-2 expression. View Full-Text
Keywords: skin microbiota; actinic keratosis; squamous cell carcinoma; basal cell carcinoma; Staphylococcus aureus; antimicrobial peptides; human β-defensin skin microbiota; actinic keratosis; squamous cell carcinoma; basal cell carcinoma; Staphylococcus aureus; antimicrobial peptides; human β-defensin
Show Figures

Graphical abstract

MDPI and ACS Style

Madhusudhan, N.; Pausan, M.R.; Halwachs, B.; Durdević, M.; Windisch, M.; Kehrmann, J.; Patra, V.; Wolf, P.; Boukamp, P.; Moissl-Eichinger, C.; Cerroni, L.; Becker, J.C.; Gorkiewicz, G. Molecular Profiling of Keratinocyte Skin Tumors Links Staphylococcus aureus Overabundance and Increased Human β-Defensin-2 Expression to Growth Promotion of Squamous Cell Carcinoma. Cancers 2020, 12, 541. https://doi.org/10.3390/cancers12030541

AMA Style

Madhusudhan N, Pausan MR, Halwachs B, Durdević M, Windisch M, Kehrmann J, Patra V, Wolf P, Boukamp P, Moissl-Eichinger C, Cerroni L, Becker JC, Gorkiewicz G. Molecular Profiling of Keratinocyte Skin Tumors Links Staphylococcus aureus Overabundance and Increased Human β-Defensin-2 Expression to Growth Promotion of Squamous Cell Carcinoma. Cancers. 2020; 12(3):541. https://doi.org/10.3390/cancers12030541

Chicago/Turabian Style

Madhusudhan, Nandhitha; Pausan, Manuela R.; Halwachs, Bettina; Durdević, Marija; Windisch, Markus; Kehrmann, Jan; Patra, VijayKumar; Wolf, Peter; Boukamp, Petra; Moissl-Eichinger, Christine; Cerroni, Lorenzo; Becker, Jürgen C.; Gorkiewicz, Gregor. 2020. "Molecular Profiling of Keratinocyte Skin Tumors Links Staphylococcus aureus Overabundance and Increased Human β-Defensin-2 Expression to Growth Promotion of Squamous Cell Carcinoma" Cancers 12, no. 3: 541. https://doi.org/10.3390/cancers12030541

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop