MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. miR-181 Family Is Downregulated in CD11b+ Monocytes Extracted from Glioblastoma
2.2. MiR-181a Regulates the Survival of Immune-Competent Mice Harboring GL261 Brain Tumors
2.3. Identification and Validation of miR-181a-5p Apoptotic Targets
3. Discussion
4. Materials and Methods
4.1. Isolation of CD14b+ Cells from Blood and CD11b+ Cells from Glioblastoma Tissue
4.2. RNA Extraction and Quantitative Real-Time PCR
4.3. Monocyte Culture and Differentiation
4.4. Quantification of OPN Using Enzyme-Linked Immunosorbent Assay
4.5. Cell Culture
4.6. Transient and Stable Transfections of miRNAs
4.7. Luciferase Assay
4.8. BrdU Proliferation Assay
4.9. Apoptosis Assay
4.10. Caspase-3 Activity
4.11. Syngeneic Intracranial Glioma Model
4.12. In Vivo Treatments
4.13. Statistical Analysis
4.14. Approval & Ethical Statement
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Marisetty, A.; Wei, J.; Kong, L.-Y.; Ott, M.; Fang, D.; Sabbagh, A.; Heimberger, A.B. MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme. Cancers 2020, 12, 3813. https://doi.org/10.3390/cancers12123813
Marisetty A, Wei J, Kong L-Y, Ott M, Fang D, Sabbagh A, Heimberger AB. MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme. Cancers. 2020; 12(12):3813. https://doi.org/10.3390/cancers12123813
Chicago/Turabian StyleMarisetty, Anantha, Jun Wei, Ling-Yuan Kong, Martina Ott, Dexing Fang, Aria Sabbagh, and Amy B. Heimberger. 2020. "MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme" Cancers 12, no. 12: 3813. https://doi.org/10.3390/cancers12123813