Neural Networks Recapitulation by Cancer Cells Promotes Disease Progression: A Novel Role of p73 Isoforms in Cancer-Neuronal Crosstalk
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Phenotype-Driven Identification of Genes Essential for Normal Nervous System Development and Neurological Function
2.2. Nervous System-Related Genes Are Frequently Mutated in Cancer Patient Tumors and Enriched in Cancer Driver Genes
2.3. Nervous System-Related Genes Are Preferentially Upregulated in Aggressive Stages and Predict Patient Outcomes in a Cancer Type-Dependent Manner
2.4. Different Cancer Types Co-Opt Genes Essential for the Development of Distinct Anatomical Parts of the Nervous System
2.5. Genes Involved in Several Stages of Neurogenesis and Neuronal Differentiation are Reactivated during Cancer Progression
2.6. Neurogenesis and Neoneurogenesis Mediators Are Abundantly Expressed during Melanoma Progression
2.7. Neurogenesis, Neoneurogenesis and Stemness Factors Are Concomitantly Regulated by TP73-Derived Isoforms
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Adenovector Generation and Transduction
4.3. RNA Isolation and q-PCR
4.4. Western Blot
4.5. Identification of Nervous System-Related Genes
4.6. Gene Expression Analysis in CCLE Database
4.7. Kaplan–Meier Survival Analysis of TCGA Patient Data
4.8. Neurodifferentiation Gene Expression Data Analysis
4.8.1. Transcriptomic Data Retrieval
4.8.2. Differential Gene Expression Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Logotheti, S.; Marquardt, S.; Richter, C.; Sophie Hain, R.; Murr, N.; Takan, I.; Pavlopoulou, A.; Pützer, B.M. Neural Networks Recapitulation by Cancer Cells Promotes Disease Progression: A Novel Role of p73 Isoforms in Cancer-Neuronal Crosstalk. Cancers 2020, 12, 3789. https://doi.org/10.3390/cancers12123789
Logotheti S, Marquardt S, Richter C, Sophie Hain R, Murr N, Takan I, Pavlopoulou A, Pützer BM. Neural Networks Recapitulation by Cancer Cells Promotes Disease Progression: A Novel Role of p73 Isoforms in Cancer-Neuronal Crosstalk. Cancers. 2020; 12(12):3789. https://doi.org/10.3390/cancers12123789
Chicago/Turabian StyleLogotheti, Stella, Stephan Marquardt, Christin Richter, Renée Sophie Hain, Nico Murr, Işıl Takan, Athanasia Pavlopoulou, and Brigitte M. Pützer. 2020. "Neural Networks Recapitulation by Cancer Cells Promotes Disease Progression: A Novel Role of p73 Isoforms in Cancer-Neuronal Crosstalk" Cancers 12, no. 12: 3789. https://doi.org/10.3390/cancers12123789