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Open AccessArticle

The EGFR-TMEM167A-p53 Axis Defines the Aggressiveness of Gliomas

1
Neurooncology Unit, Instituto de Salud Carlos III-UFIEC, Crtra/Majadahonda-Pozuelo, Km 2, Majadahonda, 28220 Madrid, Spain
2
Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), 28220 Madrid, Spain
3
Fundación Andrés Marcio “Niños contra la laminopatía”, 28220 Madrid, Spain
4
Gene Therapy Unit, Instituto de Salud Carlos III-IIER, 28220 Madrid, Spain
5
Instituto de investigaciones Biomédicas I + 12, Hospital 12 de Octubre, 28220 Madrid, Spain
6
Instituto Cajal, Centro Superior de Investigaciones Científicas (CSIC), 28220 Madrid, Spain
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(1), 208; https://doi.org/10.3390/cancers12010208
Received: 7 December 2019 / Revised: 3 January 2020 / Accepted: 8 January 2020 / Published: 14 January 2020
(This article belongs to the Special Issue Tumors of the Central Nervous System: An Update)
Despite the high frequency of EGFR and TP53 genetic alterations in gliomas, little is known about their crosstalk during tumor progression. Here, we described a mutually exclusive distribution between mutations in these two genes. We found that wild-type p53 gliomas are more aggressive than their mutant counterparts, probably because the former accumulate amplifications and/or mutations in EGFR and show a stronger activation of this receptor. In addition, we identified a series of genes associated with vesicular trafficking of EGFR in p53 wild-type gliomas. Among these genes, TMEM167A showed the strongest implication in overall survival in this group of tumors. In agreement with this observation, inhibition of TMEM167A expression impaired the subcutaneous and the intracranial growth of wild-type p53 gliomas, regardless of the presence of EGFR mutations. In the absence of p53 mutations, TMEM167A knockdown reduced the acidification of intracellular vesicles, affecting the autophagy process and impairing EGFR trafficking and signaling. This effect was mimicked by an inhibitor of the vacuolar ATPase. We propose that the increased aggressiveness of wild-type p53 gliomas might be due to the increase in growth factor signaling activity, which depends on the regulation of vesicular trafficking by TMEM167A. View Full-Text
Keywords: glioma; wild-type p53; mutant p53; EGFR/AKT signaling; vesicular trafficking; autophagy glioma; wild-type p53; mutant p53; EGFR/AKT signaling; vesicular trafficking; autophagy
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Segura-Collar, B.; Gargini, R.; Tovar-Ambel, E.; Hernández-SanMiguel, E.; Epifano, C.; Pérez de Castro, I.; Hernández-Laín, A.; Casas-Tintó, S.; Sánchez-Gómez, P. The EGFR-TMEM167A-p53 Axis Defines the Aggressiveness of Gliomas. Cancers 2020, 12, 208.

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