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Electrochemotherapy Causes Caspase-Independent Necrotic-Like Death in Pancreatic Cancer Cells

Cancer Research at UCC, Western Gateway Building, University College Cork, T12 XF62 Cork, Ireland
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Cancers 2019, 11(8), 1177; https://doi.org/10.3390/cancers11081177
Received: 25 June 2019 / Revised: 29 July 2019 / Accepted: 2 August 2019 / Published: 14 August 2019
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Abstract

Pancreatic cancer represents a major challenge in oncology. Poor permeability of the pancreas and resistance to currently available therapies are impediments to improved patient survival. By transiently increasing cell membrane porosity and increasing drug uptake, Electrochemotherapy (ECT) has the potential to overcome these issues. In this study, we have evaluated the response of human and murine pancreatic cancer cells, in vitro, to electroporation in combination with Bleomycin, Cisplatin, or Oxaliplatin (ECT). The cytotoxic actions of all three drugs are potentiated when combined with electroporation in these cells. The biochemical and morphological changes post ECT are associated with immunogenic cell death that occurs with necroptosis rather than apoptosis. Moreover, ECT-induced cell death is rescued by Nec-1 suggesting that necroptosis may play a role in cell death mediated by cancer therapies. View Full-Text
Keywords: ECT; necroptosis; apoptosis; ICD; necrostatin-1 ECT; necroptosis; apoptosis; ICD; necrostatin-1
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Fernandes, P.; O’Donovan, T.R.; McKenna, S.L.; Forde, P.F. Electrochemotherapy Causes Caspase-Independent Necrotic-Like Death in Pancreatic Cancer Cells. Cancers 2019, 11, 1177.

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