Protein Kinase A Distribution in Meningioma
Abstract
:1. Introduction
2. Results
2.1. Meningioma Tissue Binds cAMP
2.2. PKA Regulatory and Catalytic Subunits Immunofluorescence
2.3. PKA RI is Extremely Labile in Meningioma
2.4. Correlation of PKA Catalytic Subunit Expression Levels with Different Gene Products
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. Tissue Processing
4.3. Image Analysis
4.4. Expression Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
8-Fluo-cAMP | 8-(2-fluoresceinylthioureidoaminoethylthio)-adenosine 3’,5’ -cyclic monophosphate |
Alexa488-cAMP | 8-(Alexa488)- adenosine 3’,5’-cyclic monophosphate |
Alexa555-cAMP | 8-(Alexa555)- adenosine 3’,5’-cyclic monophosphate |
cAMP | cyclic AMP |
CNS | Central nervous system |
PBS | Phosphate buffered saline |
PKA | Protein kinase A, cAMP-dependent protein kinase |
R | Regulatory subunits of cAMP dependent protein kinases |
SAF-cAMP | 8-(5-thioacetamidofluorescein)-adenosine 3’,5’-cyclic monophosphate |
SAF-cGMP | 8-(5-thioacetamidofluorescein)-guanosin 3’,5’-cyclic monophosphate |
SAR-cAMP | 8-(5-thioacetamidotetramethylrhodamine)-adenosine 3’,5’-cyclic monophosphate |
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CASE N° | cAMP equilibrium binding | PKA RI IF | PKA RII IF | PKA CATALYTIC IF |
---|---|---|---|---|
1 | + | + Mostly on vessels | +++ | +++ |
2 | +/− Only in some zones | + | +++ Only in some zones | +++ Only in some zones |
3 | +++ | + | +++ | +++ |
4 | ++ | + | +++ | +++ |
5 | ++ | ++ | ++ | ++ |
6 | + | + | ++ | ++ |
7 | + | NA | NA | ++ |
8 | ++ | + | +++ | +++ |
9 | - | + | ++ Only in some zones | ++ Only in some zones |
10 | + | + Only in some zones | +++ | +++ |
11 | +/− | - | +++ | +++ |
12 | +++ | + | +++ | +++ |
13 | +++ | - | +++ | +++ |
PRKACA | PRKAR1A | PRKAR1B | PRKAR2A | PRKAR2B | |
---|---|---|---|---|---|
NF2 | 0.2994 ** | 0.0679 | −0.0406 | 0.0965 | 0.1524 |
CDC2 | −0.2973 ** | 0.0518 | −0.0246 | 0.1128 | −0.3763 *** |
MLF1IP | −0.2908 ** | 0.0363 | −0.0844 | 0.2692 * | −0.3098 ** |
CKS2 | −0.3188 ** | −0.1402 | −0.1074 | 0.0945 | −0.3754 *** |
PRC1 | −0.3622 *** | −0.0497 | −0.0640 | 0.1630 | −0.3563 *** |
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Caretta, A.; Denaro, L.; D’Avella, D.; Mucignat-Caretta, C. Protein Kinase A Distribution in Meningioma. Cancers 2019, 11, 1686. https://doi.org/10.3390/cancers11111686
Caretta A, Denaro L, D’Avella D, Mucignat-Caretta C. Protein Kinase A Distribution in Meningioma. Cancers. 2019; 11(11):1686. https://doi.org/10.3390/cancers11111686
Chicago/Turabian StyleCaretta, Antonio, Luca Denaro, Domenico D’Avella, and Carla Mucignat-Caretta. 2019. "Protein Kinase A Distribution in Meningioma" Cancers 11, no. 11: 1686. https://doi.org/10.3390/cancers11111686