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miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy

Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Author to whom correspondence should be addressed.
Cancers 2018, 10(9), 289;
Received: 17 July 2018 / Revised: 7 August 2018 / Accepted: 21 August 2018 / Published: 25 August 2018
(This article belongs to the Special Issue Nanotechnology and Cancer)
The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells. View Full-Text
Keywords: miR-205; docetaxel; prostate cancer; chemosensitivity and EMT miR-205; docetaxel; prostate cancer; chemosensitivity and EMT
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MDPI and ACS Style

Nagesh, P.K.B.; Chowdhury, P.; Hatami, E.; Boya, V.K.N.; Kashyap, V.K.; Khan, S.; Hafeez, B.B.; Chauhan, S.C.; Jaggi, M.; Yallapu, M.M. miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy. Cancers 2018, 10, 289.

AMA Style

Nagesh PKB, Chowdhury P, Hatami E, Boya VKN, Kashyap VK, Khan S, Hafeez BB, Chauhan SC, Jaggi M, Yallapu MM. miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy. Cancers. 2018; 10(9):289.

Chicago/Turabian Style

Nagesh, Prashanth K.B., Pallabita Chowdhury, Elham Hatami, Vijaya K.N. Boya, Vivek K. Kashyap, Sheema Khan, Bilal B. Hafeez, Subhash C. Chauhan, Meena Jaggi, and Murali M. Yallapu. 2018. "miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy" Cancers 10, no. 9: 289.

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