Targeting MicroRNA-143 Leads to Inhibition of Glioblastoma Tumor Progression
AbstractGlioblastoma (GBM) is the most common and aggressive of all brain tumors, with a median survival of only 14 months after initial diagnosis. Novel therapeutic approaches are an unmet need for GBM treatment. MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression at the post-transcriptional level. Several dysregulated miRNAs have been identified in all cancer types including GBM. In this study, we aimed to uncover the role of miR-143 in GBM cell lines, patient samples, and mouse models. Quantitative real-time RT-PCR of RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples showed that the relative expression of miR-143 was higher in GBM patients compared to control individuals. Transient transfection of GBM cells with a miR-143 oligonucleotide inhibitor (miR-143-inh) resulted in reduced cell proliferation, increased apoptosis, and cell cycle arrest. SLC30A8, a glucose metabolism-related protein, was identified as a direct target of miR-143 in GBM cells. Moreover, multiple injections of GBM tumor-bearing mice with a miR-143-inh-liposomal formulation significantly reduced tumor growth compared to control mice. The reduced in vitro cell growth and in vivo tumor growth following miRNA-143 inhibition suggests that miR-143 is a potential therapeutic target for GBM therapy. View Full-Text
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Lozada-Delgado, E.L.; Grafals-Ruiz, N.; Miranda-Román, M.A.; Santana-Rivera, Y.; Valiyeva, F.; Rivera-Díaz, M.; Marcos-Martínez, M.J.; Vivas-Mejía, P.E. Targeting MicroRNA-143 Leads to Inhibition of Glioblastoma Tumor Progression. Cancers 2018, 10, 382.
Lozada-Delgado EL, Grafals-Ruiz N, Miranda-Román MA, Santana-Rivera Y, Valiyeva F, Rivera-Díaz M, Marcos-Martínez MJ, Vivas-Mejía PE. Targeting MicroRNA-143 Leads to Inhibition of Glioblastoma Tumor Progression. Cancers. 2018; 10(10):382.Chicago/Turabian Style
Lozada-Delgado, Eunice L.; Grafals-Ruiz, Nilmary; Miranda-Román, Miguel A.; Santana-Rivera, Yasmarie; Valiyeva, Fatma; Rivera-Díaz, Mónica; Marcos-Martínez, María J.; Vivas-Mejía, Pablo E. 2018. "Targeting MicroRNA-143 Leads to Inhibition of Glioblastoma Tumor Progression." Cancers 10, no. 10: 382.
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