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Volume 11
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10.3390/mi11121097
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Article

Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads

by
Yin Zhang
*,
Zengdao Gu
,
Jiabin Zhao
,
Liying Shao
and
Yajing Kan
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(12), 1097; https://doi.org/10.3390/mi11121097
Submission received: 19 November 2020 / Revised: 8 December 2020 / Accepted: 10 December 2020 / Published: 11 December 2020
(This article belongs to the Special Issue Micro- and Nanofluidics for Bionanoparticle Analysis, Volume II)
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Abstract

Simple, rapid, and low-cost detection of DNA with specific sequence is crucial for molecular diagnosis and therapy applications. In this research, the target DNA molecules are bonded to the streptavidin-coated microbeads, after hybridizing with biotinylated probes. A nanopore with a diameter significantly smaller than the microbeads is used to detect DNA molecules through the ionic pulse signals. Because the DNA molecules attached on the microbead should dissociate from the beads before completely passing through the pore, the signal duration time for the target DNA is two orders of magnitude longer than free DNA. Moreover, the high local concentration of target DNA molecules on the surface of microbeads leads to multiple DNA molecules translocating through the pore simultaneously, which generates pulse signals with amplitude much larger than single free DNA translocation events. Therefore, the DNA molecules with specific sequence can be easily identified by a nanopore sensor assisted by microbeads according to the ionic pulse signals.
Keywords: nanopore; microbead; DNA detection nanopore; microbead; DNA detection

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MDPI and ACS Style

Zhang, Y.; Gu, Z.; Zhao, J.; Shao, L.; Kan, Y. Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads. Micromachines 2020, 11, 1097. https://doi.org/10.3390/mi11121097

AMA Style

Zhang Y, Gu Z, Zhao J, Shao L, Kan Y. Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads. Micromachines. 2020; 11(12):1097. https://doi.org/10.3390/mi11121097

Chicago/Turabian Style

Zhang, Yin, Zengdao Gu, Jiabin Zhao, Liying Shao, and Yajing Kan. 2020. "Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads" Micromachines 11, no. 12: 1097. https://doi.org/10.3390/mi11121097

APA Style

Zhang, Y., Gu, Z., Zhao, J., Shao, L., & Kan, Y. (2020). Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads. Micromachines, 11(12), 1097. https://doi.org/10.3390/mi11121097

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