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Micromachines 2018, 9(7), 318; https://doi.org/10.3390/mi9070318

Multiple and Periodic Measurement of RBC Aggregation and ESR in Parallel Microfluidic Channels under On-Off Blood Flow Control

1
Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Korea
2
Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea
*
Author to whom correspondence should be addressed.
Received: 16 May 2018 / Revised: 13 June 2018 / Accepted: 21 June 2018 / Published: 24 June 2018
(This article belongs to the Special Issue Micro/Nano Devices for Blood Analysis)
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Abstract

Red blood cell (RBC) aggregation causes to alter hemodynamic behaviors at low flow-rate regions of post-capillary venules. Additionally, it is significantly elevated in inflammatory or pathophysiological conditions. In this study, multiple and periodic measurements of RBC aggregation and erythrocyte sedimentation rate (ESR) are suggested by sucking blood from a pipette tip into parallel microfluidic channels, and quantifying image intensity, especially through single experiment. Here, a microfluidic device was prepared from a master mold using the xurography technique rather than micro-electro-mechanical-system fabrication techniques. In order to consider variations of RBC aggregation in microfluidic channels due to continuous ESR in the conical pipette tip, two indices (aggregation index (AI) and erythrocyte-sedimentation-rate aggregation index (EAI)) are evaluated by using temporal variations of microscopic, image-based intensity. The proposed method is employed to evaluate the effect of hematocrit and dextran solution on RBC aggregation under continuous ESR in the conical pipette tip. As a result, EAI displays a significantly linear relationship with modified conventional ESR measurement obtained by quantifying time constants. In addition, EAI varies linearly within a specific concentration of dextran solution. In conclusion, the proposed method is able to measure RBC aggregation under continuous ESR in the conical pipette tip. Furthermore, the method provides multiple data of RBC aggregation and ESR through a single experiment. A future study will involve employing the proposed method to evaluate biophysical properties of blood samples collected from cardiovascular diseases. View Full-Text
Keywords: red blood cell (RBC) aggregation; multiple microfluidic channels; master molder using xurography technique; RBC aggregation index; modified conventional erythrocyte sedimentation rate (ESR) method; regression analysis red blood cell (RBC) aggregation; multiple microfluidic channels; master molder using xurography technique; RBC aggregation index; modified conventional erythrocyte sedimentation rate (ESR) method; regression analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kang, Y.J.; Kim, B.J. Multiple and Periodic Measurement of RBC Aggregation and ESR in Parallel Microfluidic Channels under On-Off Blood Flow Control. Micromachines 2018, 9, 318.

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