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Article

Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels

1
Microelectromechanical Systems Research Unit (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal
2
Instituto Politécnico de Bragança, ESTiG, C. Sta. Apolónia, 5300-253 Bragança, Portugal
3
MEtRICs, DEM, University of Minho, 4800-058 Guimarães, Portugal
4
CEFT, University of Porto, 4000-008 Porto, Portugal
*
Author to whom correspondence should be addressed.
Micromachines 2018, 9(8), 384; https://doi.org/10.3390/mi9080384
Received: 19 June 2018 / Revised: 19 July 2018 / Accepted: 30 July 2018 / Published: 2 August 2018
(This article belongs to the Special Issue Micro/Nano Devices for Blood Analysis)
Malaria is one of the leading causes of death in underdeveloped regions. Thus, the development of rapid, efficient, and competitive diagnostic techniques is essential. This work reports a study of the deformability and velocity assessment of healthy and artificially impaired red blood cells (RBCs), with the purpose of potentially mimicking malaria effects, in narrow polydimethylsiloxane microchannels. To obtain impaired RBCs, their properties were modified by adding, to the RBCs, different concentrations of glucose, glutaraldehyde, or diamide, in order to increase the cells’ rigidity. The effects of the RBCs’ artificial stiffening were evaluated by combining image analysis techniques with microchannels with a contraction width of 8 µm, making it possible to measure the cells’ deformability and velocity of both healthy and modified RBCs. The results showed that healthy RBCs naturally deform when they cross the contractions and rapidly recover their original shape. In contrast, for the modified samples with high concentration of chemicals, the same did not occur. Additionally, for all the tested modification methods, the results have shown a decrease in the RBCs’ deformability and velocity as the cells’ rigidity increases, when compared to the behavior of healthy RBCs samples. These results show the ability of the image analysis tools combined with microchannel contractions to obtain crucial information on the pathological blood phenomena in microcirculation. Particularly, it was possible to measure the deformability of the RBCs and their velocity, resulting in a velocity/deformability relation in the microchannel. This correlation shows great potential to relate the RBCs’ behavior with the various stages of malaria, helping to establish the development of new diagnostic systems towards point-of-care devices. View Full-Text
Keywords: biomicrofluidics; red blood cells; deformability; velocity biomicrofluidics; red blood cells; deformability; velocity
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MDPI and ACS Style

Boas, L.V.; Faustino, V.; Lima, R.; Miranda, J.M.; Minas, G.; Fernandes, C.S.V.; Catarino, S.O. Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels. Micromachines 2018, 9, 384. https://doi.org/10.3390/mi9080384

AMA Style

Boas LV, Faustino V, Lima R, Miranda JM, Minas G, Fernandes CSV, Catarino SO. Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels. Micromachines. 2018; 9(8):384. https://doi.org/10.3390/mi9080384

Chicago/Turabian Style

Boas, Liliana V., Vera Faustino, Rui Lima, João M. Miranda, Graça Minas, Carla S.V. Fernandes, and Susana O. Catarino. 2018. "Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels" Micromachines 9, no. 8: 384. https://doi.org/10.3390/mi9080384

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