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Micromachines 2014, 5(1), 27-49;

Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate

Department of Applied Physics, KTH—Royal Institute of Technology, SE-106 91 Stockholm, Sweden
Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, SE-171 21 Stockholm, Sweden
Author to whom correspondence should be addressed.
Received: 19 December 2013 / Revised: 23 January 2014 / Accepted: 23 January 2014 / Published: 6 February 2014
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
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This review describes the use of ultrasound for inducing and retaining cell-cell contact in multi-well microplates combined with live-cell fluorescence microscopy. This platform has been used for studying the interaction between natural killer (NK) cells and cancer cells at the level of individual cells. The review includes basic principles of ultrasonic particle manipulation, design criteria when building a multi-well microplate device for this purpose, biocompatibility aspects, and finally, two examples of biological applications: Dynamic imaging of the inhibitory immune synapse, and studies of the heterogeneity in killing dynamics of NK cells interacting with cancer cells. View Full-Text
Keywords: ultrasound; lab-on-a-chip; acoustofluidics; acoustic trapping; natural killer cells ultrasound; lab-on-a-chip; acoustofluidics; acoustic trapping; natural killer cells

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Wiklund, M.; Christakou, A.E.; Ohlin, M.; Iranmanesh, I.; Frisk, T.; Vanherberghen, B.; Önfelt, B. Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate. Micromachines 2014, 5, 27-49.

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