Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Xu, Hongmei; Tae, Hyunhyuk; Cho, Nam-Joon; Huang, Changjin; Hsia, K. Jimmy

doi:10.3390/mi13010134

Open AccessArticle

Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

by

Hongmei Xu

¹

,

Hyunhyuk Tae

²

,

Nam-Joon Cho

^2,*

,

Changjin Huang

^1,*

and

K. Jimmy Hsia

^1,3,*

¹

School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore

²

School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore

³

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore

^*

Authors to whom correspondence should be addressed.

Micromachines 2022, 13(1), 134; https://doi.org/10.3390/mi13010134

Submission received: 22 December 2021 / Revised: 10 January 2022 / Accepted: 11 January 2022 / Published: 15 January 2022

(This article belongs to the Special Issue Lipid Bilayers on Chip, Volume II)

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Abstract

The solvent-assisted lipid bilayer (SALB) formation method provides a simple and efficient, microfluidic-based strategy to fabricate supported lipid bilayers (SLBs) with rich compositional diversity on a wide range of solid supports. While various studies have been performed to characterize SLBs formed using the SALB method, relatively limited work has been carried out to understand the underlying mechanisms of SALB formation under various experimental conditions. Through thermodynamic modeling, we studied the experimental parameters that affect the SALB formation process, including substrate surface properties, initial lipid concentration, and temperature. It was found that all the parameters are critically important to successfully form high-quality SLBs. The model also helps to identify the range of parameter space within which conformal, homogeneous SLBs can be fabricated, and provides mechanistic guidance to optimize experimental conditions for lipid membrane-related applications.

Keywords: supported lipid bilayer; thermodynamics; solvent exchange; self-assembly; SALB

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MDPI and ACS Style

Xu, H.; Tae, H.; Cho, N.-J.; Huang, C.; Hsia, K.J. Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process. Micromachines 2022, 13, 134. https://doi.org/10.3390/mi13010134

AMA Style

Xu H, Tae H, Cho N-J, Huang C, Hsia KJ. Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process. Micromachines. 2022; 13(1):134. https://doi.org/10.3390/mi13010134

Chicago/Turabian Style

Xu, Hongmei, Hyunhyuk Tae, Nam-Joon Cho, Changjin Huang, and K. Jimmy Hsia. 2022. "Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process" Micromachines 13, no. 1: 134. https://doi.org/10.3390/mi13010134

APA Style

Xu, H., Tae, H., Cho, N.-J., Huang, C., & Hsia, K. J. (2022). Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process. Micromachines, 13(1), 134. https://doi.org/10.3390/mi13010134

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

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