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Review

Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

by 1,†, 1,†, 1, 2 and 1,*
1
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
2
Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing 100191, China
*
Author to whom correspondence should be addressed.
Equal contribution.
Academic Editors: Francesco Gentile and Gobind Das
Micromachines 2021, 12(5), 492; https://doi.org/10.3390/mi12050492
Received: 6 April 2021 / Revised: 21 April 2021 / Accepted: 23 April 2021 / Published: 27 April 2021
(This article belongs to the Special Issue Advances in Biomedical Nanotechnology)
Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules. View Full-Text
Keywords: FRET; smFRET; biomolecule; nucleic acid structure; protein FRET; smFRET; biomolecule; nucleic acid structure; protein
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MDPI and ACS Style

Qiao, Y.; Luo, Y.; Long, N.; Xing, Y.; Tu, J. Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules. Micromachines 2021, 12, 492. https://doi.org/10.3390/mi12050492

AMA Style

Qiao Y, Luo Y, Long N, Xing Y, Tu J. Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules. Micromachines. 2021; 12(5):492. https://doi.org/10.3390/mi12050492

Chicago/Turabian Style

Qiao, Yi, Yuhan Luo, Naiyun Long, Yi Xing, and Jing Tu. 2021. "Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules" Micromachines 12, no. 5: 492. https://doi.org/10.3390/mi12050492

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