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Micromachines
Volume 11
Issue 9
10.3390/mi11090856
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Communication

Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

by
Yifei Ye
1,2,
Xiaofeng Luan
1,2,
Lingqian Zhang
1,
Wenjie Zhao
1,2,
Jie Cheng
1,2,
Mingxiao Li
1,
Yang Zhao
1,* and
Chengjun Huang
1,2,*
1
R&D Center of Healthcare Electronics, Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, China
2
School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(9), 856; https://doi.org/10.3390/mi11090856
Submission received: 17 August 2020 / Revised: 8 September 2020 / Accepted: 14 September 2020 / Published: 16 September 2020
(This article belongs to the Special Issue Microfluidics for Cells and Other Organisms, Volume II)
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Abstract

The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporation and simultaneous impedance monitoring in a constriction microchannel. When single cells (A549) were continuously passing through the constriction microchannel, a localized high electric field was applied on the cell membrane, which resulted in highly efficient (up to 96.6%) electroporation. During a single cell entering the constriction channel, an abrupt impedance drop was noticed and demonstrated to be correlated with the occurrence of electroporation. Besides, while the cell was moving in the constriction channel, the stabilized impedance showed the capability to quantify the electroporation extent. The correspondence of the impedance variation and electroporation was validated by the intracellular delivery of the fluorescence indicator (propidium iodide). Based on the obtained results, this system is capable of precise control of electroporation and real-time, label-free impedance assessment, providing a potential tool for intracellular delivery and other biomedical applications.
Keywords: microfluidics; intracellular cargo delivery; single-cell electroporation; electroporation effect assessment microfluidics; intracellular cargo delivery; single-cell electroporation; electroporation effect assessment

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MDPI and ACS Style

Ye, Y.; Luan, X.; Zhang, L.; Zhao, W.; Cheng, J.; Li, M.; Zhao, Y.; Huang, C. Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel. Micromachines 2020, 11, 856. https://doi.org/10.3390/mi11090856

AMA Style

Ye Y, Luan X, Zhang L, Zhao W, Cheng J, Li M, Zhao Y, Huang C. Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel. Micromachines. 2020; 11(9):856. https://doi.org/10.3390/mi11090856

Chicago/Turabian Style

Ye, Yifei, Xiaofeng Luan, Lingqian Zhang, Wenjie Zhao, Jie Cheng, Mingxiao Li, Yang Zhao, and Chengjun Huang. 2020. "Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel" Micromachines 11, no. 9: 856. https://doi.org/10.3390/mi11090856

APA Style

Ye, Y., Luan, X., Zhang, L., Zhao, W., Cheng, J., Li, M., Zhao, Y., & Huang, C. (2020). Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel. Micromachines, 11(9), 856. https://doi.org/10.3390/mi11090856

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