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Open AccessFeature PaperArticle

Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging

1
Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea
2
Department of Nano Manufacturing Technology, Korea Institute of Machinery & Materials (KIMM), Daejeon 34103, Korea
3
School of Materials Science and Engineering, University of Ulsan, Ulsan 44776, Korea
4
Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea
5
Department of Physics, Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea
6
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea
*
Author to whom correspondence should be addressed.
Authors equally contributed.
Micromachines 2020, 11(6), 582; https://doi.org/10.3390/mi11060582
Received: 19 May 2020 / Revised: 5 June 2020 / Accepted: 8 June 2020 / Published: 10 June 2020
(This article belongs to the Special Issue Micro/Nano Fabrication for Life Sciences)
3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures. View Full-Text
Keywords: micromirror; microfabrication; 3D imaging; Optical MEMS micromirror; microfabrication; 3D imaging; Optical MEMS
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MDPI and ACS Style

Lee, D.; Kim, J.; Song, E.; Jeong, J.-Y.; Jeon, E.-C.; Kim, P.; Lee, W. Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging. Micromachines 2020, 11, 582.

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