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Open AccessArticle

Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

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Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
2
AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
3
Immunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(1), 107; https://doi.org/10.3390/mi11010107
Received: 10 December 2019 / Revised: 8 January 2020 / Accepted: 15 January 2020 / Published: 19 January 2020
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis, Volume II)
Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell. View Full-Text
Keywords: localized surface plasmon resonance (LSPR) technology; Interleukin 6 (IL-6) detection; single cell trapping; single cell level immunoassay localized surface plasmon resonance (LSPR) technology; Interleukin 6 (IL-6) detection; single cell trapping; single cell level immunoassay
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Zhu, C.; Luo, X.; Espulgar, W.V.; Koyama, S.; Kumanogoh, A.; Saito, M.; Takamatsu, H.; Tamiya, E. Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology. Micromachines 2020, 11, 107.

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