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Open AccessArticle

Characterization of Single-Nucleus Electrical Properties by Microfluidic Constriction Channel

by Hongyan Liang 1,2,†, Yi Zhang 1,2,†, Deyong Chen 1,2, Huiwen Tan 1,2, Yu Zheng 3, Junbo Wang 1,2,* and Jian Chen 1,2,*
1
State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100094, China
2
School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 101408, China
3
Shandong University, Jinan 250100, China
*
Authors to whom correspondence should be addressed.
Co-first authors.
Micromachines 2019, 10(11), 740; https://doi.org/10.3390/mi10110740
Received: 27 September 2019 / Revised: 26 October 2019 / Accepted: 29 October 2019 / Published: 31 October 2019
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis, Volume II)
As key bioelectrical markers, equivalent capacitance (Cne, i.e., capacitance per unit area) and resistance (Rne, i.e., resistivity multiply thickness) of nuclear envelopes have emerged as promising electrical indicators, which cannot be effectively measured by conventional approaches. In this study, single nuclei were isolated from whole cells and trapped at the entrances of microfluidic constriction channels, and then corresponding impedance profiles were sampled and translated into single-nucleus Cne and Rne based on a home-developed equivalent electrical model. Cne and Rne of A549 nuclei were first quantified as 3.43 ± 1.81 μF/cm2 and 2.03 ± 1.40 Ω·cm2 (Nn = 35), which were shown not to be affected by variations of key parameters in nuclear isolation and measurement. The developed approach in this study was also used to measure a second type of nuclei, producing Cne and Rne of 3.75 ± 3.17 μF/cm2 and 1.01 ± 0.70 Ω·cm2 for SW620 (Nn = 17). This study may provide a new perspective in single-cell electrical characterization, enabling cell type classification and cell status evaluation based on bioelectrical markers of nuclei. View Full-Text
Keywords: microfluidics; single-nucleus analysis; constriction channel; electrical properties; nuclear envelope microfluidics; single-nucleus analysis; constriction channel; electrical properties; nuclear envelope
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Liang, H.; Zhang, Y.; Chen, D.; Tan, H.; Zheng, Y.; Wang, J.; Chen, J. Characterization of Single-Nucleus Electrical Properties by Microfluidic Constriction Channel. Micromachines 2019, 10, 740.

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