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Article

Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps

1
Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan
2
Department of Engineering Design, Indian Institute of Technology Madras, Tamil Nadu 600036, India
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(4), 442; https://doi.org/10.3390/mi11040442
Received: 21 March 2020 / Revised: 17 April 2020 / Accepted: 22 April 2020 / Published: 22 April 2020
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis, Volume II)
High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. The flow was bidirectionally controlled by changing the DC bias. A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The application of DC-biased AC voltage (100 kHz, 10 Vpp, and VDC: −4 V) provided a sufficient electroosmotic flow outside the nozzle array. This integration method of nozzle and pumps is anticipated to be a standard integration method. The operating conditions of DC-biased AC electrokinetic pumps in a biological buffer was clarified and found useful for cell manipulation. View Full-Text
Keywords: micronozzle-array; parallel cell manipulation; bidirectional electrokinetic pump; DC biased AC electrokinetic flow micronozzle-array; parallel cell manipulation; bidirectional electrokinetic pump; DC biased AC electrokinetic flow
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MDPI and ACS Style

Nagai, M.; Kato, K.; Soga, S.; Santra, T.S.; Shibata, T. Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps. Micromachines 2020, 11, 442. https://doi.org/10.3390/mi11040442

AMA Style

Nagai M, Kato K, Soga S, Santra TS, Shibata T. Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps. Micromachines. 2020; 11(4):442. https://doi.org/10.3390/mi11040442

Chicago/Turabian Style

Nagai, Moeto, Keita Kato, Satoshi Soga, Tuhin S. Santra, and Takayuki Shibata. 2020. "Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps" Micromachines 11, no. 4: 442. https://doi.org/10.3390/mi11040442

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