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Open AccessArticle

Cord-Based Microfluidic Chips as A Platform for ELISA and Glucose Assays

Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032-8202, USA
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Micromachines 2019, 10(9), 614; https://doi.org/10.3390/mi10090614
Received: 4 August 2019 / Revised: 3 September 2019 / Accepted: 13 September 2019 / Published: 15 September 2019
(This article belongs to the Special Issue 10th Anniversary of Micromachines)
This paper describes the development and application of microfluidic cord-based analytical devices (µCADs) in two enzyme-linked immunosorbent assays (ELISAs) and glucose assay. In this study, biotinylated goat anti-mouse immunoglobulin (IgG) antibody, rabbit IgG antibody, and glucose are quantitatively detected. In the ELISA systems, the antibody is spotted on the cord at the detection site and a series of washes, followed by streptavidin-alkaline phosphatase (Strep-ALP) or alkaline phosphatase (ALP)-conjugated secondary antibody and colorimetric substrate, completing the experiment. The devices are subsequently scanned and analyzed yielding a correlation between inverse yellow or inverse blue intensity and antibody concentration. For the first ELISA, a linear range of detection was observed at lower concentrations (2.50 × 10−4–1.75 × 10−3 mg/mL) of Strep-ALP with saturation of the enzyme achieved at higher concentrations (>2.50 × 10−4). For the second ELISA, the L50 was demonstrated to be 167.6 fmol/zone. The glucose assay consisted of spotting increasing concentrations of glucose on the analysis sites and transporting, via capillary action, a solution containing glucose oxidase (GOx), horseradish peroxidase (HRP), and potassium iodide (KI) to the detection sites realizing a yellow-brown color indicating oxidation of iodide to iodine. The device was then dried, scanned, and analyzed to show the correlation between yellow inverse intensity and glucose. Glucose in artificial urine showed good correlation using the devices. View Full-Text
Keywords: enzyme-linked immunosorbent assay; microfluidics; microfluidics cord-based analytical device; point-of-care diagnostic device enzyme-linked immunosorbent assay; microfluidics; microfluidics cord-based analytical device; point-of-care diagnostic device
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MDPI and ACS Style

Elomaa, J.; Gallegos, L.; Gomez, F.A. Cord-Based Microfluidic Chips as A Platform for ELISA and Glucose Assays. Micromachines 2019, 10, 614.

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