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Tangential Flow Microfiltration for Viral Separation and Concentration

1
Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015, USA
2
Department of Bioengineering, Lehigh University, Bethlehem, PA 18015, USA
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(5), 320; https://doi.org/10.3390/mi10050320
Received: 26 April 2019 / Revised: 7 May 2019 / Accepted: 9 May 2019 / Published: 12 May 2019
(This article belongs to the Special Issue Micro- and Nanofluidics for Bionanoparticle Analysis)
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Abstract

Microfluidic devices that allow biological particle separation and concentration have found wide applications in medical diagnosis. Here we present a viral separation polydimethylsiloxane (PDMS) device that combines tangential flow microfiltration and affinity capture to enrich HIV virus in a single flow-through fashion. The set-up contains a filtration device and a tandem resistance channel. The filtration device consists of two parallel flow channels separated by a polycarbonate nanoporous membrane. The resistance channel, with dimensions design-guided by COMSOL simulation, controls flow permeation through the membrane in the filtration device. A flow-dependent viral capture efficiency is observed, which likely reflects the interplay of several processes, including specific binding of target virus, physical deposition of non-specific particles, and membrane cleaning by shear flow. At the optimal flow rate, nearly 100% of viral particles in the permeate are captured on the membrane with various input viral concentrations. With its easy operation and consistent performance, this microfluidic device provides a potential solution for HIV sample preparation in resource-limited settings. View Full-Text
Keywords: HIV diagnostics; cross-flow filtration; microfluidic device; COMSOL; nanoporous membrane HIV diagnostics; cross-flow filtration; microfluidic device; COMSOL; nanoporous membrane
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Wang, Y.; Keller, K.; Cheng, X. Tangential Flow Microfiltration for Viral Separation and Concentration. Micromachines 2019, 10, 320.

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