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Tangential Flow Microfiltration for Viral Separation and Concentration

Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015, USA
Department of Bioengineering, Lehigh University, Bethlehem, PA 18015, USA
Author to whom correspondence should be addressed.
Micromachines 2019, 10(5), 320;
Received: 26 April 2019 / Revised: 7 May 2019 / Accepted: 9 May 2019 / Published: 12 May 2019
(This article belongs to the Special Issue Micro- and Nanofluidics for Bionanoparticle Analysis)
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Microfluidic devices that allow biological particle separation and concentration have found wide applications in medical diagnosis. Here we present a viral separation polydimethylsiloxane (PDMS) device that combines tangential flow microfiltration and affinity capture to enrich HIV virus in a single flow-through fashion. The set-up contains a filtration device and a tandem resistance channel. The filtration device consists of two parallel flow channels separated by a polycarbonate nanoporous membrane. The resistance channel, with dimensions design-guided by COMSOL simulation, controls flow permeation through the membrane in the filtration device. A flow-dependent viral capture efficiency is observed, which likely reflects the interplay of several processes, including specific binding of target virus, physical deposition of non-specific particles, and membrane cleaning by shear flow. At the optimal flow rate, nearly 100% of viral particles in the permeate are captured on the membrane with various input viral concentrations. With its easy operation and consistent performance, this microfluidic device provides a potential solution for HIV sample preparation in resource-limited settings. View Full-Text
Keywords: HIV diagnostics; cross-flow filtration; microfluidic device; COMSOL; nanoporous membrane HIV diagnostics; cross-flow filtration; microfluidic device; COMSOL; nanoporous membrane

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Wang, Y.; Keller, K.; Cheng, X. Tangential Flow Microfiltration for Viral Separation and Concentration. Micromachines 2019, 10, 320.

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