Tangential Flow Microfiltration for Viral Separation and Concentration
AbstractMicrofluidic devices that allow biological particle separation and concentration have found wide applications in medical diagnosis. Here we present a viral separation polydimethylsiloxane (PDMS) device that combines tangential flow microfiltration and affinity capture to enrich HIV virus in a single flow-through fashion. The set-up contains a filtration device and a tandem resistance channel. The filtration device consists of two parallel flow channels separated by a polycarbonate nanoporous membrane. The resistance channel, with dimensions design-guided by COMSOL simulation, controls flow permeation through the membrane in the filtration device. A flow-dependent viral capture efficiency is observed, which likely reflects the interplay of several processes, including specific binding of target virus, physical deposition of non-specific particles, and membrane cleaning by shear flow. At the optimal flow rate, nearly 100% of viral particles in the permeate are captured on the membrane with various input viral concentrations. With its easy operation and consistent performance, this microfluidic device provides a potential solution for HIV sample preparation in resource-limited settings. View Full-Text
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Wang, Y.; Keller, K.; Cheng, X. Tangential Flow Microfiltration for Viral Separation and Concentration. Micromachines 2019, 10, 320.
Wang Y, Keller K, Cheng X. Tangential Flow Microfiltration for Viral Separation and Concentration. Micromachines. 2019; 10(5):320.Chicago/Turabian Style
Wang, Yi; Keller, Keely; Cheng, Xuanhong. 2019. "Tangential Flow Microfiltration for Viral Separation and Concentration." Micromachines 10, no. 5: 320.
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