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Microfluidics for Artificial Life: Techniques for Bottom-Up Synthetic Biology

Department of Chemistry, White City Campus, Imperial College London, London SW7 2AZ, UK
fabriCELL, Imperial College London, London SW7 2AZ, UK
Department of Chemistry, King’s College London, Britannia House, London SE1 1DB, UK
Author to whom correspondence should be addressed.
Micromachines 2019, 10(5), 299;
Received: 15 February 2019 / Revised: 24 April 2019 / Accepted: 26 April 2019 / Published: 30 April 2019
(This article belongs to the Special Issue Microfluidic Applications in Synthetic Biology)
Synthetic biology is a rapidly growing multidisciplinary branch of science that exploits the advancement of molecular and cellular biology. Conventional modification of pre-existing cells is referred to as the top-down approach. Bottom-up synthetic biology is an emerging complementary branch that seeks to construct artificial cells from natural or synthetic components. One of the aims in bottom-up synthetic biology is to construct or mimic the complex pathways present in living cells. The recent, and rapidly growing, application of microfluidics in the field is driven by the central tenet of the bottom-up approach—the pursuit of controllably generating artificial cells with precisely defined parameters, in terms of molecular and geometrical composition. In this review we survey conventional methods of artificial cell synthesis and their limitations. We proceed to show how microfluidic approaches have been pivotal in overcoming these limitations and ushering in a new generation of complexity that may be imbued in artificial cells and the milieu of applications that result. View Full-Text
Keywords: microfluidics; synthetic biology; bottom-up microfluidics; synthetic biology; bottom-up
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Supramaniam, P.; Ces, O.; Salehi-Reyhani, A. Microfluidics for Artificial Life: Techniques for Bottom-Up Synthetic Biology. Micromachines 2019, 10, 299.

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