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Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths

1
Department of Chemistry, Iowa State University, 1605 Gilman Hall, 2415 Osborn Drive, Ames, IA 50011, USA
2
Department of Mechanical Engineering, Iowa State University, 2043 Black Engineering, 2529 Union Drive, Ames, IA 50011, USA
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(4), 271; https://doi.org/10.3390/mi10040271
Received: 9 March 2019 / Revised: 16 April 2019 / Accepted: 18 April 2019 / Published: 23 April 2019
(This article belongs to the Special Issue AC Electrokinetics in Microfluidic Devices)
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Abstract

Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dimensions of each electrode in an array of wireless bipolar electrodes (BPEs). The cells are captured by dielectrophoresis (DEP), which confers selectivity, and patterns cells without the need for physical barriers or adhesive interactions that can alter cell function. Our findings demonstrate that this approach readily achieves fine control of cell cluster size over a broader range set by other experimental parameters. These parameters include the magnitude of the voltage applied externally to drive capture at the BPE array, the rate of fluid flow, and the time allowed for DEP-based cell capture. Therefore, the reported method is anticipated to allow the influence of cluster size on cell function to be more fully investigated. View Full-Text
Keywords: cell patterning; cell cluster; dielectrophoresis; wireless electrode array; bipolar electrode cell patterning; cell cluster; dielectrophoresis; wireless electrode array; bipolar electrode
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Banovetz, J.T.; Li, M.; Pagariya, D.; Kim, S.; Ganapathysubramanian, B.; Anand, R.K. Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths. Micromachines 2019, 10, 271.

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