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A Continuous Flow-through Microfluidic Device for Electrical Lysis of Cells

Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan
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Micromachines 2019, 10(4), 247; https://doi.org/10.3390/mi10040247
Received: 31 March 2019 / Revised: 10 April 2019 / Accepted: 11 April 2019 / Published: 13 April 2019
(This article belongs to the Special Issue AC Electrokinetics in Microfluidic Devices)
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Abstract

In contrast to the delicate 3D electrodes in the literature, a simple flow-through device is proposed here for continuous and massive lysis of cells using electricity. The device is essentially a rectangular microchannel with a planar electrode array built on its bottom wall, actuated by alternating current (AC) voltages between neighboring electrodes, and can be incorporated easily into other biomedical systems. Human whole blood diluted 10 times with phosphate-buffered saline (about 6 × 108 cells per mL) was pumped through the device, and the cells were completely lysed within 7 s after the application of a 20 V peak-to-peak voltage at 1 MHz, up to 400 μL/hr. Electric field and Maxwell stress were calculated for assessing electrical lysis. Only the lower half-channel was exposed to an electric field exceeding the irreversible threshold value of cell electroporation (Eth2), suggesting that a cross flow, proposed here primarily as the electro-thermally induced flow, was responsible for bringing the cells in the upper half-channel downward to the lower half-channel. The Maxwell shear stress associated with Eth2 was one order of magnitude less than the threshold mechanical stresses for lysis, implying that an applied moderate mechanical stress could aid electrical lysis. View Full-Text
Keywords: electrical cell lysis; continuous flow-through device; human red blood cells; Maxwell stress electrical cell lysis; continuous flow-through device; human red blood cells; Maxwell stress
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Lo, Y.-J.; Lei, U. A Continuous Flow-through Microfluidic Device for Electrical Lysis of Cells. Micromachines 2019, 10, 247.

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