Next Article in Journal
Design and Fabrication of a Wavelength-Selective Near-Infrared Metasurface Emitter for a Thermophotovoltaic System
Next Article in Special Issue
Deformation of a Red Blood Cell in a Narrow Rectangular Microchannel
Previous Article in Journal
Interactive Effects of Rarefaction and Surface Roughness on Aerodynamic Lubrication of Microbearings
Previous Article in Special Issue
Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device
Open AccessArticle

Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells

Department of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(2), 156; https://doi.org/10.3390/mi10020156
Received: 29 January 2019 / Revised: 19 February 2019 / Accepted: 22 February 2019 / Published: 25 February 2019
(This article belongs to the Special Issue Micro/Nano Devices for Blood Analysis)
Some cells cultured in vitro have multiple nuclei. Since cultured cells are used in various fields of science, including tissue engineering, the nature of the multinucleated cells must be determined. However, multinucleated cells are not frequently observed. In this study, a method to efficiently obtain multinucleated cells was established and their morphological properties were investigated. Initially, we established conditions to quickly and easily generate multinucleated cells by seeding a Xenopus tadpole epithelium tissue-derived cell line (XTC-YF) on less and more hydrophilic dishes, and incubating the cultures with medium supplemented with or without Y-27632—a ROCK inhibitor—to reduce cell contractility. Notably, 88% of the cells cultured on a less hydrophilic dish in medium supplemented with Y-27632 became multinucleate 48 h after seeding, whereas less than 5% of cells cultured under other conditions exhibited this morphology. Some cells showed an odd number (three and five) of cell nuclei 72 h after seeding. Multinucleated cells displayed a significantly smaller nuclear area, larger cell area, and smaller nuclear circularity. As changes in the morphology of the cells correlated with their functions, the proposed method would help researchers understand the functions of multinucleated cells. View Full-Text
Keywords: multinucleated cells; XTC-YF cells; morphological analysis; Y-27632; hydrophobic dish multinucleated cells; XTC-YF cells; morphological analysis; Y-27632; hydrophobic dish
Show Figures

Graphical abstract

MDPI and ACS Style

Sugita, S.; Munechika, R.; Nakamura, M. Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells. Micromachines 2019, 10, 156.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop