Next Article in Journal
Liquid Cladding Mediated Optical Fiber Sensors for Copper Ion Detection
Next Article in Special Issue
Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells
Previous Article in Journal
Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures
Previous Article in Special Issue
Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
Article

Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device

1
School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, China
2
Integrated Chinese and Western Medicine Postdoctoral research station, Jinan University, Guangzhou 510632, China
3
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
4
College of Stomatology, Dalian Medical University, Dalian 116024, China
*
Authors to whom correspondence should be addressed.
These authors have equally contributed to this work.
Micromachines 2018, 9(9), 470; https://doi.org/10.3390/mi9090470
Received: 26 July 2018 / Revised: 7 September 2018 / Accepted: 10 September 2018 / Published: 17 September 2018
(This article belongs to the Special Issue Micro/Nano Devices for Blood Analysis)
Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker used in clinical applications. The primarily clinical method for measuring CEA is based on chemiluminescence in serum during enzyme-linked immunosorbent assays (ELISA) in 96-well plates. However, this multi-step process requires large and expensive instruments, and takes a long time. In this study, a high-throughput centrifugal microfluidic device was developed for detecting CEA in serum without the need for cumbersome washing steps normally used in immunoreactions. This centrifugal microdevice contains 14 identical pencil-like units, and the CEA molecules are separated from the bulk serum for subsequent immunofluorescence detection using density gradient centrifugation in each unit simultaneously. To determine the optimal conditions for CEA detection in serum, the effects of the density of the medium, rotation speed, and spin duration were investigated. The measured values from 34 clinical serum samples using this high-throughput centrifugal microfluidic device showed good agreement with the known values (average relative error = 9.22%). These results indicate that the high-throughput centrifugal microfluidic device could provide an alternative approach for replacing the classical method for CEA detection in clinical serum samples. View Full-Text
Keywords: centrifugal microfluidic device; CEA detection; density medium; fluorescent chemiluminescence centrifugal microfluidic device; CEA detection; density medium; fluorescent chemiluminescence
Show Figures

Figure 1

MDPI and ACS Style

Gao, Z.; Chen, Z.; Deng, J.; Li, X.; Qu, Y.; Xu, L.; Luo, Y.; Lu, Y.; Liu, T.; Zhao, W.; Lin, B. Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device. Micromachines 2018, 9, 470. https://doi.org/10.3390/mi9090470

AMA Style

Gao Z, Chen Z, Deng J, Li X, Qu Y, Xu L, Luo Y, Lu Y, Liu T, Zhao W, Lin B. Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device. Micromachines. 2018; 9(9):470. https://doi.org/10.3390/mi9090470

Chicago/Turabian Style

Gao, Zhigang, Zongzheng Chen, Jiu Deng, Xiaorui Li, Yueyang Qu, Lingling Xu, Yong Luo, Yao Lu, Tingjiao Liu, Weijie Zhao, and Bingcheng Lin. 2018. "Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device" Micromachines 9, no. 9: 470. https://doi.org/10.3390/mi9090470

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop