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The Effect of Microfluidic Geometry on Myoblast Migration

Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA
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Micromachines 2019, 10(2), 143; https://doi.org/10.3390/mi10020143
Received: 10 December 2018 / Revised: 4 February 2019 / Accepted: 19 February 2019 / Published: 21 February 2019
(This article belongs to the Special Issue Microfluidic Cell Assay Chips)
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Abstract

In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels—from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5–20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration. View Full-Text
Keywords: microfluidics; myoblasts; migration; PDMS; microfabrication microfluidics; myoblasts; migration; PDMS; microfabrication
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Atmaramani, R.; Black, B.J.; Lam, K.H.; Sheth, V.M.; Pancrazio, J.J.; Schmidtke, D.W.; Alsmadi, N.Z. The Effect of Microfluidic Geometry on Myoblast Migration. Micromachines 2019, 10, 143.

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