Next Article in Journal
A Review of Design and Fabrication of the Bionic Flapping Wing Micro Air Vehicles
Next Article in Special Issue
Engineering Microfluidic Organoid-on-a-Chip Platforms
Previous Article in Journal
Correction: Shen, T. et al. High-Precision and Low-Cost Wireless 16-Channel Measurement System for Malachite Green Detection. Micromachines, 2018, 9, 646
Previous Article in Special Issue
Development of Microfluidic Stretch System for Studying Recovery of Damaged Skeletal Muscle Cells
Open AccessArticle

The Effect of Microfluidic Geometry on Myoblast Migration

Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(2), 143; https://doi.org/10.3390/mi10020143
Received: 10 December 2018 / Revised: 4 February 2019 / Accepted: 19 February 2019 / Published: 21 February 2019
(This article belongs to the Special Issue Microfluidic Cell Assay Chips)
In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels—from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5–20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration. View Full-Text
Keywords: microfluidics; myoblasts; migration; PDMS; microfabrication microfluidics; myoblasts; migration; PDMS; microfabrication
Show Figures

Figure 1

MDPI and ACS Style

Atmaramani, R.; Black, B.J.; Lam, K.H.; Sheth, V.M.; Pancrazio, J.J.; Schmidtke, D.W.; Alsmadi, N.Z. The Effect of Microfluidic Geometry on Myoblast Migration. Micromachines 2019, 10, 143. https://doi.org/10.3390/mi10020143

AMA Style

Atmaramani R, Black BJ, Lam KH, Sheth VM, Pancrazio JJ, Schmidtke DW, Alsmadi NZ. The Effect of Microfluidic Geometry on Myoblast Migration. Micromachines. 2019; 10(2):143. https://doi.org/10.3390/mi10020143

Chicago/Turabian Style

Atmaramani, Rahul; Black, Bryan J.; Lam, Kevin H.; Sheth, Vinit M.; Pancrazio, Joseph J.; Schmidtke, David W.; Alsmadi, Nesreen Z. 2019. "The Effect of Microfluidic Geometry on Myoblast Migration" Micromachines 10, no. 2: 143. https://doi.org/10.3390/mi10020143

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop