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Open AccessArticle

A Phenolic Acid Decarboxylase-Based All-Enzyme Hydrogel for Flow Reactor Technology

1
Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), 76187 Karlsruhe, Germany
2
Institute for Mechanical Process Engineering and Mechanics (MVM), Karlsruhe Institute of Technology (KIT), 76187 Karlsruhe, Germany
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(12), 795; https://doi.org/10.3390/mi10120795
Received: 18 October 2019 / Revised: 15 November 2019 / Accepted: 18 November 2019 / Published: 20 November 2019
Carrier-free enzyme immobilization techniques are an important development in the field of efficient and streamlined continuous synthetic processes using microreactors. Here, the use of monolithic, self-assembling all-enzyme hydrogels is expanded to phenolic acid decarboxylases. This provides access to the continuous flow production of p-hydroxystyrene from p-coumaric acid for more than 10 h with conversions ≥98% and space time yields of 57.7 g·(d·L)−1. Furthermore, modulation of the degree of crosslinking in the hydrogels resulted in a defined variation of the rheological behavior in terms of elasticity and mesh size of the corresponding materials. This work is addressing the demand of sustainable strategies for defunctionalization of renewable feedstocks. View Full-Text
Keywords: biocatalysis; phenolic acid decarboxylase; hydrogel; enzyme immobilization; SpyCatcher/SpyTag; microreactor biocatalysis; phenolic acid decarboxylase; hydrogel; enzyme immobilization; SpyCatcher/SpyTag; microreactor
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MDPI and ACS Style

Mittmann, E.; Gallus, S.; Bitterwolf, P.; Oelschlaeger, C.; Willenbacher, N.; Niemeyer, C.M.; Rabe, K.S. A Phenolic Acid Decarboxylase-Based All-Enzyme Hydrogel for Flow Reactor Technology. Micromachines 2019, 10, 795.

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