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Open AccessArticle

Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis

Institute for Biological Interfaces (IBG1), Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany
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Micromachines 2019, 10(11), 783; https://doi.org/10.3390/mi10110783
Received: 18 October 2019 / Revised: 8 November 2019 / Accepted: 13 November 2019 / Published: 15 November 2019
All-enzyme hydrogels are biocatalytic materials, with which various enzymes can be immobilized in microreactors in a simple, mild, and efficient manner to be used for continuous flow processes. Here we present the construction and application of a cofactor regenerating hydrogel based on the imine reductase GF3546 from Streptomyces sp. combined with the cofactor regenerating glucose-1-dehydrogenase from Bacillus subtilis. The resulting hydrogel materials were characterized in terms of binding kinetics and viscoelastic properties. The materials were formed by rapid covalent crosslinking in less than 5 min, and they showed a typical mesh size of 67 ± 2 nm. The gels were applied for continuous flow biocatalysis. In a microfluidic reactor setup, the hydrogels showed excellent conversions of imines to amines for up to 40 h in continuous flow mode. Variation of flow rates led to a process where the gels showed a maximum space-time-yield of 150 g·(L·day)−1 at 100 μL/min. View Full-Text
Keywords: enzyme immobilization; continuous; biocatalysis; imine reductase; microreactor enzyme immobilization; continuous; biocatalysis; imine reductase; microreactor
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MDPI and ACS Style

Bitterwolf, P.; Ott, F.; Rabe, K.S.; Niemeyer, C.M. Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis. Micromachines 2019, 10, 783.

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