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Open AccessArticle

Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement

1
Institute of Complex Systems, Bioelectronics (ICS-8), Forschungszentrum Jülich, 52428 Jülich, Germany
2
Faculty of Mathematics, Computer Science and Natural Sciences, RWTH Aachen University, 52076 Aachen, Germany
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(10), 659; https://doi.org/10.3390/mi10100659
Received: 29 August 2019 / Revised: 23 September 2019 / Accepted: 25 September 2019 / Published: 30 September 2019
(This article belongs to the Special Issue New Approaches to Micropatterning)
Patterned neuronal cell cultures are important tools for investigating neuronal signal integration, network function, and cell–substrate interactions. Because of the variable nature of neuronal cells, the widely used coating method of microcontact printing is in constant need of improvements and adaptations depending on the pattern, cell type, and coating solutions available for a certain experimental system. In this work, we report on three approaches to modify microcontact printing on borosilicate glass surfaces, which we evaluate with contact angle measurements and by determining the quality of patterned neuronal growth. Although background toxification with manganese salt does not result in the desired pattern enhancement, a simple heat treatment of the glass substrates leads to improved background hydrophobicity and therefore neuronal patterning. Thirdly, we extended a microcontact printing process based on covalently linking the glass surface and the coating molecule via an epoxysilane. This extension is an additional hydrophobization step with dodecylamine. We demonstrate that shelf life of the silanized glass is at least 22 weeks, leading to consistently reliable neuronal patterning by microcontact printing. Thus, we compared three practical additions to microcontact printing, two of which can easily be implemented into a workflow for the investigation of patterned neuronal networks. View Full-Text
Keywords: microcontact printing; patterned neuronal networks; epoxysilane modification; tempering; Glymo; (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) microcontact printing; patterned neuronal networks; epoxysilane modification; tempering; Glymo; (3-Glycidyloxypropyl)trimethoxysilane (GPTMS)
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Hondrich, T.J.J.; Deußen, O.; Grannemann, C.; Brinkmann, D.; Offenhäusser, A. Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement. Micromachines 2019, 10, 659.

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