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Open AccessArticle

Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device

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Ph.D’s Degree Program in Industrial Engineering, Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand
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Industrial Engineering Department, Chiang Mai University, Chiang Mai 50200, Thailand
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Synchrotron Light Research Institute (Public Organization), 111 University Avenue, Nakhon Ratchasima 30000, Thailand
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(10), 647; https://doi.org/10.3390/mi10100647
Received: 21 August 2019 / Revised: 11 September 2019 / Accepted: 23 September 2019 / Published: 26 September 2019
In this research, we aimed to count the ratio of the number of motile to immotile sperm for patients with infertility problems based on a low-sperm-concentration examination. The microfluidic system consists of two series of applications: The conventional separation of motile sperm and the proposed inductance (L), capacitance (C), and resistance (R) or LCR impedance sperm counter. In the experiment, 96% of motile sperm were isolated from nonmotile sperm in the first part and transported to the second part to count and calculate real-time sperm concentration. A pair of microelectrodes composed of thin metal film were integrated between microchannels, resulting in a peak signal for LCR single-cell detection, as well as the estimated total sperm concentration. A minimum of 10 µL of the sperm sample was completely analyzed with an accuracy of 94.8% compared with the standard computer-assisted semen analysis (CASA) method. This method could be applied for low-cost sperm separation and counting in the future. View Full-Text
Keywords: real-time monitoring; sperm; motile sperm; LCR impedance; single cell counting; microfluidic system real-time monitoring; sperm; motile sperm; LCR impedance; single cell counting; microfluidic system
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Phiphattanaphiphop, C.; Leksakul, K.; Phatthanakun, R.; Suthummapiwat, A. Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device. Micromachines 2019, 10, 647.

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