Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device
Abstract
:1. Introduction
2. Materials and Methods
2.1. Design and Fabrication
2.2. Sample
2.3. Impedance Detection and Analysis
2.3.1. Overview of LCR Impedance Program
2.3.2. Detection Method for LCR Impedance and Counting Spermatozoa
2.3.3. Programming and GUI
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Source | DF | SS | MS | F | p |
---|---|---|---|---|---|
Factor | 1 | 4,435,877,635 | 24,435,877,635 | 20,697.51 | 0.000 |
Error | 6 | 7,083,715 | 1,180,619 | - | - |
Total | 7 | 24,442,961,351 | - | - | - |
S = 1087R2 = 99.97% R2 (adj) = 99.97% |
Sample | Baseline Impedance (kΩ) | Standard Error | Count/90 s |
---|---|---|---|
1 | 112.48 | 1.76% | 37 |
2 | 110.49 | 0.05% | 42 |
3 | 110.47 | 0.06% | 32 |
4 | 108.72 | 1.65% | 40 |
Average | 110.54 | 0.88% | 38 |
Analysis | Volume (µL) | Time (min) | Total No. of Sperm Analyzed | Method | Validation (100%) |
---|---|---|---|---|---|
CASA | 10 | 15–20 | 517 | Image Programming | Reference Standard 100% |
LCR Impedance | 10 | 5–10 | 488 | Difference in Impedance | 94.8% |
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Phiphattanaphiphop, C.; Leksakul, K.; Phatthanakun, R.; Suthummapiwat, A. Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device. Micromachines 2019, 10, 647. https://doi.org/10.3390/mi10100647
Phiphattanaphiphop C, Leksakul K, Phatthanakun R, Suthummapiwat A. Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device. Micromachines. 2019; 10(10):647. https://doi.org/10.3390/mi10100647
Chicago/Turabian StylePhiphattanaphiphop, Chalinee, Komgrit Leksakul, Rungrueang Phatthanakun, and Apirak Suthummapiwat. 2019. "Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device" Micromachines 10, no. 10: 647. https://doi.org/10.3390/mi10100647