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Open AccessCommunication

Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1

1
Department of Biochemistry and Molecular Biology, University of Valladolid, 47005 Valladolid, Spain
2
Bioforge, University of Valladolid, Spain and Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 47002 Valladolid, Spain
3
Department of Nutrition and Bromatology, University of Valladolid, 47005 Valladolid, Spain
4
Department of Pharmacy & Pharmaceutical Technology and University Institute of Industrial Pharmacy (IUFI), Complutense University of Madrid, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Pang-Chui Shaw
Toxins 2016, 8(6), 184; https://doi.org/10.3390/toxins8060184
Received: 14 April 2016 / Revised: 24 May 2016 / Accepted: 3 June 2016 / Published: 10 June 2016
(This article belongs to the Collection Immunotoxins 2016)
Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT)—containing recombinant musarmin 1 (single chain ribosome-inactivating proteins) linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 × 10−10 to 10−9 M. View Full-Text
Keywords: recombinant musarmin; endoglin; CD105; 44G4; anti-endoglin monoclonal antibody; anti-tumor therapy; cancer therapy recombinant musarmin; endoglin; CD105; 44G4; anti-endoglin monoclonal antibody; anti-tumor therapy; cancer therapy
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MDPI and ACS Style

Barriuso, B.; Antolín, P.; Arias, F.J.; Girotti, A.; Jiménez, P.; Cordoba-Diaz, M.; Cordoba-Diaz, D.; Girbés, T. Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1. Toxins 2016, 8, 184. https://doi.org/10.3390/toxins8060184

AMA Style

Barriuso B, Antolín P, Arias FJ, Girotti A, Jiménez P, Cordoba-Diaz M, Cordoba-Diaz D, Girbés T. Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1. Toxins. 2016; 8(6):184. https://doi.org/10.3390/toxins8060184

Chicago/Turabian Style

Barriuso, Begoña; Antolín, Pilar; Arias, F. J.; Girotti, Alessandra; Jiménez, Pilar; Cordoba-Diaz, Manuel; Cordoba-Diaz, Damián; Girbés, Tomás. 2016. "Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1" Toxins 8, no. 6: 184. https://doi.org/10.3390/toxins8060184

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