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Keywords = recombinant musarmin

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10 pages, 1393 KiB  
Communication
Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1
by Begoña Barriuso, Pilar Antolín, F. Javier Arias, Alessandra Girotti, Pilar Jiménez, Manuel Cordoba-Diaz, Damián Cordoba-Diaz and Tomás Girbés
Toxins 2016, 8(6), 184; https://doi.org/10.3390/toxins8060184 - 10 Jun 2016
Cited by 8 | Viewed by 5577
Abstract
Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to [...] Read more.
Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT)—containing recombinant musarmin 1 (single chain ribosome-inactivating proteins) linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 × 10−10 to 10−9 M. Full article
(This article belongs to the Collection Immunotoxins 2016)
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