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AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea

Department of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan
Authors to whom correspondence should be addressed.
Academic Editor: Anton Meinhart
Toxins 2016, 8(6), 174;
Received: 22 March 2016 / Accepted: 30 May 2016 / Published: 4 June 2016
(This article belongs to the Special Issue Toxin-Antitoxin System in Bacteria)
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Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions. View Full-Text
Keywords: Nitrosomonas europaea; toxin-antitoxin system; MazF; sequence-specific endoribonuclease Nitrosomonas europaea; toxin-antitoxin system; MazF; sequence-specific endoribonuclease

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Miyamoto, T.; Yokota, A.; Tsuneda, S.; Noda, N. AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea. Toxins 2016, 8, 174.

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