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Article

Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry

1
Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea
2
Food Contaminants Division, National Institute of Food & Drug Safety Evaluation, Osong 28159, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Aldo Laganà
Toxins 2016, 8(12), 371; https://doi.org/10.3390/toxins8120371
Received: 5 October 2016 / Revised: 5 December 2016 / Accepted: 6 December 2016 / Published: 13 December 2016
(This article belongs to the Special Issue LC-MS/MS Method for Mycotoxin Analysis)
An interlaboratory study was performed in eight laboratories to validate a liquid chromatography–tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of aflatoxins and sterigmatocystin (STC) in white rice and sorghum (Sorghum bicolor). Fortified samples (at three different levels) of white rice and sorghum were extracted, purified through a solid-phase extraction (SPE) column, and then analyzed by LC/MS/MS. The apparent recoveries (ARs) ranged from 78.8% to 95.0% for aflatoxins and from 85.3% to 96.7% for STC. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of aflatoxins were in the ranges 7.9%–33.8% and 24.4%–81.0%, respectively. For STC, the RSDr ranged from 7.1% to 40.2% and the RSDR ranged from 28.1% to 99.2%. The Horwitz ratio values for the aflatoxins and STC ranged from 0.4 to 1.2 in white rice and from 0.3 to 1.0 in sorghum, respectively. These results validated this method for the simultaneous determination of aflatoxins and STC by LC/MS/MS after SPE column cleanup. The percentages of satisfactory Z-score values (|Z| ≤ 2) were the following: for white rice, 100% for aflatoxins and STC; for sorghum, 100%, except in data from two laboratories for STC (0.3 μg/kg). This validated that the LC/MS/MS method was successfully applied for the determination of aflatoxins and STC in 20 white rice and 20 sorghum samples sourced from Korean markets. View Full-Text
Keywords: aflatoxins; sterigmatocystin; simultaneous determination; liquid chromatography–tandem mass spectrometry aflatoxins; sterigmatocystin; simultaneous determination; liquid chromatography–tandem mass spectrometry
MDPI and ACS Style

Ok, H.E.; Tian, F.; Hong, E.Y.; Paek, O.; Kim, S.-H.; Kim, D.; Chun, H.S. Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry. Toxins 2016, 8, 371. https://doi.org/10.3390/toxins8120371

AMA Style

Ok HE, Tian F, Hong EY, Paek O, Kim S-H, Kim D, Chun HS. Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry. Toxins. 2016; 8(12):371. https://doi.org/10.3390/toxins8120371

Chicago/Turabian Style

Ok, Hyun Ee, Fei Tian, Eun Young Hong, Ockjin Paek, Sheen-Hee Kim, Dongsul Kim, and Hyang Sook Chun. 2016. "Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry" Toxins 8, no. 12: 371. https://doi.org/10.3390/toxins8120371

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