Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques
Abstract
:1. Introduction
2. Results
2.1. Proteome Analysis
2.2. Peptidome Analysis
3. Discussion
4. Materials and Methods
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Spot no. # | Identified Protein & | Accession * | Organism ¥ | Mass [kDa] £ | S ± | Peptide Sequence ¤ |
---|---|---|---|---|---|---|
1 | Snake venom 5′-nucleotidase | V5NTD_CROAD | Crotalus adamanteus | 65.2 | 97 | ETPVLSNPEGPYLEFR (1719.099) |
V5NTD_CROAD | Crotalus adamanteus | 65.2 | 39 | QAFEHSVHR (1110.651) | ||
V5NTD_GLOBB | Gloydius blomhoffii blomhoffii | 6 | 115 | SFELTILHTNDVMAR (1753.091) | ||
V5NTD_GLOBR | Gloydius brevicaudus | 65 | 65 | PMF SC 13.3% | ||
2 | l-amino-acid oxidase | OXLA_GLOHA | Gloydius halys | 57.4 | 116 | ETDYEEFLEIAR (1514.762) |
OXLA_PSEAU | Pseudechis austarlis | 59 | 54 | PMS SC 10.4% | ||
3 | Beta-fibrinogenase brevinase | VSPB_GLOBL | Gloydius blomhoffii | 26.3 | 65 | VIGGDECNINEHR (1512.767) |
Beta-fibrinogenase | VSPBF_MACLB | Macrovipera lebetina | 28.2 | 26 | FFCLSSK (888.452) | |
4 | Thrombin-like enzyme bilineobin | VSP2_AGKBI | Agkistrodon bilineatus | 27.1 | 64 | IIGGDECNINEHR (1526.785) |
28 | NSEHIAPLSLPSSPPIVGSVCR (2317.179) | |||||
Thrombin-like enzyme asperase | VSPL_BOTAS | Bothrops asper | 28.6 | 118 | ETYPDVPHCANINILDHAVCR (2494.238) | |
Snake venom serine protease PA | VSPP_TRIST | Trimeresurus stajnegri | 28.6 | 40 | VVLNEDEQIR (1115.567) | |
Snake venom serine proteinase pallabin | VSP1_GLOHA | Gloydius halys | 29.3 | 27 | LDSPVKNSAHIAPLSLPSSPPVGSDCR (2888.600) | |
Snake venom serine proteinase 9 | VSP9_CROAD | Crotalus adamanteus | 11.7 | 164 | ETYPDVPHCANINILDYEVCR (2578.230) | |
Snake venom serine proteinase 12 | VSPC_CROAD | Crotalus adamanteus | 29.3 | 113 | DIMLIRLDSPVSNSEHIAPLSLPSSPPSVGSVCR (1823.985) | |
Thrombin-like enzyme crotalase | VSPCR_CROAD | Crotalus adamanteus | 30.1 | 36 | WDKDIMLIR(1189.656) | |
5 | Protein C activator | VSPCA_AGKCO | Agkistrodon contortrix contortrix | 25.7 | 82 | PMF SC 34,2% |
91 | NSAHIAPLSLPSNPPSVGSVCR (2260.075) | |||||
VSPCA_AGKBI | Agkistrodon bilineatus | 2.2 | 68 | VVGGDECNINEHR (1498.711) | ||
6 | Basic phospholipase A2 homolog | PA2HB_AGKPI | Agkistrodon piscivorus piscivorus | 14.7 | 80 | PMF SC 49% |
Basic phospholipase A2 homolog MT1 | PA2H1_AGKCL | Agkistrodon contrortrix laticinctus | 16.5 | 80 | PMF SC 43% | |
7 | Cysteine-rich venom protein piscivorin | CRVP_AGKPI | Agkistrodon piscivorus piscivorus | 27.5 | 102 | MEHYPEAAANAER (1537.689) |
76 | MEHYPEAAANAER (1553.669) | |||||
8 | Snake venom metalloproteinase ACLF | VM1A_AGKCL | Agkistrodon piscivorus loucostroma | 47.1 | 67 | YVELVIIADHR (1327.857) |
29 | SHDNAQLLATAIVFDGIIGR (2169.126) | |||||
VM1A_AGKCL | Agkistrodon contortrix latiematus | 26.7 | 77 | YVELVIVADHR (1313.725 ) | ||
Zinc metalloproteinase disintegrin | VM2AB_AGKCO | Agkistordon contortrix contortrix | 55.1 | 185 | ISHDNAQLLTAIELDGETIGLANR (2564.272 ) | |
41 | YIELVVVADHR (1313, 713) | |||||
Snake venom metalloproteinase VMP1 | VM1V1_AGKPL | Agkistrodon piscivorus leucostoma | 47.1 | 136 | SHDNAQLLTAIVFDEGIIGR (2169.058) | |
37 | APLAGMCDPNR (1201.591) | |||||
43 | YVELVIVADHR (1327.784) | |||||
Zinc metalloproteinase disintegrin-like HR1a | VM3HA_PROFL | Protobothrops flavoviridis | 70.9 | 59 | TWVYEIVNTLNEIYR (1912.993) | |
Snake venom metalloproteinase fibrolase | VM1F_AGKCO | Akgistrodon contortrix contortrix | 23.2 | 42 | YVQLVIVADHR (1312.613) | |
9 | Acidic phospholipase A2 BpirPLA2-I | PA2A1_BOTPI | Bothrops pirajai | 14.4 | 48 | CCFVMDCCYGK (1505.585) |
49 | QICECDR (980.433) | |||||
Acidic phospholipase A2 S1E6-b | PA2AB_CALRH | Calloselasma rhodostoma | 14.3 | 56 | PMF SC 30.2% | |
Acidic phospholipase A2 | PA2A_GLOHA | Gloydius halys | 14.7 | 57 | PMF SC 25.8 % | |
Acidic phospholipase A2 1 | PA2A1_PROFL | Protobothrops flavoviridis | 15.5 | 30 | AAAICFR (808.334) | |
10 | C-type lectin APL | LECG_AGKPI | Agkistrodon piscivorus piscivorus | 16.7 | 103 | PMF SC 51.1% |
54 | DFSWEWTDR (1241.611) | |||||
105 | EFCVELVSLTGYR (1572.785) | |||||
101 | GQAEVWIGLWDK (1401.639) | |||||
C- type lectin PAL | LECG_BITAR | Bitis arietans | 16.6 | 56 | PMF SC 58.5% |
Parent ion m/z | Identified Protein & | Accession * | Organism ¥ | Peptide Sequence ≠ | Mass [Da] £ | S ± |
---|---|---|---|---|---|---|
1063.5343 | Bradykinin inhibitor peptide | BKIP_AGKBI | Agkistrodon bilineatus | TPPAGPDVGPR | 1063 | 9 |
1214.6465 | Bradykinin-potentiating peptide POL-236 | BPP36_CROAT | Crotalus atrox | QLWPRPQIPP- + Gln- > pyro-Glu (N-term Q) | 1231 | 59 |
1230.6420 | Bradykinin potentiating peptide E | gi|229310 | Gloydius blomhoffii | EKWDPPPVSPP- + Glu- > pyro-Glu (N-term E) | 1248 | 13 |
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Bocian, A.; Urbanik, M.; Hus, K.; Łyskowski, A.; Petrilla, V.; Andrejčáková, Z.; Petrillová, M.; Legáth, J. Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques. Toxins 2016, 8, 372. https://doi.org/10.3390/toxins8120372
Bocian A, Urbanik M, Hus K, Łyskowski A, Petrilla V, Andrejčáková Z, Petrillová M, Legáth J. Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques. Toxins. 2016; 8(12):372. https://doi.org/10.3390/toxins8120372
Chicago/Turabian StyleBocian, Aleksandra, Małgorzata Urbanik, Konrad Hus, Andrzej Łyskowski, Vladimír Petrilla, Zuzana Andrejčáková, Monika Petrillová, and Jaroslav Legáth. 2016. "Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques" Toxins 8, no. 12: 372. https://doi.org/10.3390/toxins8120372