Survey on Ochratoxin A Occurrence in Cured Meat Products in The Netherlands
Abstract
1. Introduction
2. Results and Discussion
2.1. Fit-for-Purpose Validation
Additional In-House Validation
2.2. Survey Samples
3. Conclusions
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Samples
4.3. Extraction and Clean-Up
4.4. LC-MS/MS
4.5. Fit-for-Purpose Validation
4.6. Data Evaluation
4.7. Quality Control
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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| Sample No | Concentration in Unspiked Sample, µg/kg | Results Corrected for Natural Contamination | Recovered Concentrations (µg/kg), Recoveries (%), and Corresponding Repeatability (RSDr, %) at Fit-for-Purpose Validation | ||
|---|---|---|---|---|---|
| At 0.2 µg/kg | At 1 µg/kg | At 5 µg/kg | |||
| 1 | 0.14 | YES | 0.211 | 0.9733 | 5.002 |
| 2 | 0.07 | YES | 0.206 | 0.996 | 5.008 |
| 3 | 0.06 | YES | 0.207 | 1.016 | 4.971 |
| 4 | <0.04 | NO | 0.219 | 0.992 | 4.859 |
| 5 | 23.04 | No result | |||
| 6 | 0.04 | YES | 0.200 | 0.984 | 5.277 |
| Recovery average | 104.3 | 99.2 | 100.5 | ||
| RSDr | 3.4 | 1.6 | 3.1 | ||
| Product Category | Number of Samples | Samples ≤ LOQ (0.04 µg/kg) | Samples Reporting Limit ≥ LOQ (0.04 µg/kg) | Samples ≥ Reporting Limit (0.2 µg/kg) | Mean Concentration ± SD (µg/kg) |
|---|---|---|---|---|---|
| Dry-cured ham | n = 17 | n = 10, 59% | n = 3, 18% | n = 4, 24% | 11.6 ± 30.1 |
| Salami and fermented sausages | n = 14 | n = 9, 64% | n = 4, 29% | n = 1, 7% | 0.14 ± 0.15 |
| Other fermented sausages | n = 10 | n = 3, 30% | n = 6, 60% | n = 1, 10% | 0.08 ± 0.07 |
| Whole muscle cured meats | n = 4 | n = 0, 0% | n = 4, 100% | n = 0, 0% | <0.04 |
| Other products | n = 5 | n = 4, 80% | n = 1, 20% | n = 0, 0% | 0.18 ± 0.00 |
| Total | n = 50 | n = 30, 60% | n = 14, 28% | n = 6, 12% | 4.1 ± 17.8 |
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Sopel, M.M.; van den Top, H.; Grzetic Martens, J.; de Nijs, M. Survey on Ochratoxin A Occurrence in Cured Meat Products in The Netherlands. Toxins 2026, 18, 262. https://doi.org/10.3390/toxins18060262
Sopel MM, van den Top H, Grzetic Martens J, de Nijs M. Survey on Ochratoxin A Occurrence in Cured Meat Products in The Netherlands. Toxins. 2026; 18(6):262. https://doi.org/10.3390/toxins18060262
Chicago/Turabian StyleSopel, Marta Magdalena, Hester van den Top, Josipa Grzetic Martens, and Monique de Nijs. 2026. "Survey on Ochratoxin A Occurrence in Cured Meat Products in The Netherlands" Toxins 18, no. 6: 262. https://doi.org/10.3390/toxins18060262
APA StyleSopel, M. M., van den Top, H., Grzetic Martens, J., & de Nijs, M. (2026). Survey on Ochratoxin A Occurrence in Cured Meat Products in The Netherlands. Toxins, 18(6), 262. https://doi.org/10.3390/toxins18060262
