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Volume 17
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10.3390/toxins17090426
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins

by
Jie Wang
1,†,
Wangzhuo Fu
1,†,
Xuezhen Ma
1,
Lin Chen
1,
Weitao Song
1,
Sumei Ling
1,
Hongyun Qian
2,
Shihua Wang
1,* and
Zhenhong Zhuang
1,*
1
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, Proteomic Research Center, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Fujian Baimeng Medical Technology Company Limited, Fuzhou 350000, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Toxins 2025, 17(9), 426; https://doi.org/10.3390/toxins17090426
Submission received: 16 June 2025 / Revised: 14 August 2025 / Accepted: 18 August 2025 / Published: 22 August 2025
(This article belongs to the Special Issue Detection, Biosynthesis and Control of Mycotoxins (4th Edition))
Versions Notes

Abstract

Due to the severe hazard of aflatoxins (AFs) to humans, it is of great significance to detect the key aflatoxins, aflatoxin B1 (AFB1) and aflatoxin G1 (AFG1), in food and feed in simple, rapid, and semi-quantitative ways. The hybridoma clone 3A1 was prepared in this study, and anti-AFB1 monoclonal antibody (mAb) with high specificity and affinity (9.38 × 108 L/mol) from 3A1 was purified. The indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) demonstrated that the linear detection range for AFB1 was 0.029–1.526 ng/mL with a limits of determination (LOD) of 0.023 ng/mL. A latex microsphere-based immunochromatographic test strip (LM-ICTS) was constructed based on 3A1, which showed that the strip could detect AFB1 (LOD: lower than 1.79 ng/mL) and AFG1 (LOD: lower than 8.08 ng/mL), and the linear detection ranges for AFB1 and AFG1 are 1.79–48.46 ng/mL and 8.08–107.40 ng/mL, respectively. The average recoveries of intra-assay and inter-assay for peanuts were (98.4 ± 4.7)% and (92.6 ± 7.6)%, and the average coefficient of variation (CVs) were 4.38% and 8.15%, respectively. For sunflower seeds, the intra-assay and inter-assay recoveries were (94.4 ± 7.2)% and (89.2 ± 4.3)%, and the average CVs were 6.6% and 4.9%, respectively. In summary, the developed LM-ICTS exhibited excellent sensitivity and specificity, which provided a rapidly stable on-site detection choice for AFB1 and AFG1 to contaminated agricultural samples, including grain and feed.
Keywords: hybridoma; AFB1; AFG1; latex microsphere; immunochromatographic test strip hybridoma; AFB1; AFG1; latex microsphere; immunochromatographic test strip

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MDPI and ACS Style

Wang, J.; Fu, W.; Ma, X.; Chen, L.; Song, W.; Ling, S.; Qian, H.; Wang, S.; Zhuang, Z. Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins. Toxins 2025, 17, 426. https://doi.org/10.3390/toxins17090426

AMA Style

Wang J, Fu W, Ma X, Chen L, Song W, Ling S, Qian H, Wang S, Zhuang Z. Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins. Toxins. 2025; 17(9):426. https://doi.org/10.3390/toxins17090426

Chicago/Turabian Style

Wang, Jie, Wangzhuo Fu, Xuezhen Ma, Lin Chen, Weitao Song, Sumei Ling, Hongyun Qian, Shihua Wang, and Zhenhong Zhuang. 2025. "Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins" Toxins 17, no. 9: 426. https://doi.org/10.3390/toxins17090426

APA Style

Wang, J., Fu, W., Ma, X., Chen, L., Song, W., Ling, S., Qian, H., Wang, S., & Zhuang, Z. (2025). Development of Latex Microsphere-Based Immunochromatographic Strips for Detecting Key Aflatoxins. Toxins, 17(9), 426. https://doi.org/10.3390/toxins17090426

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