Next Article in Journal
Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China
Next Article in Special Issue
Deoxynivalenol Degradation by Various Microbial Communities and Its Impacts on Different Bacterial Flora
Previous Article in Journal
The Effect of Foliar Fungicide and Insecticide Application on the Contamination of Fumonisins, Moniliformin and Deoxynivalenol in Maize Used for Food Purposes
Previous Article in Special Issue
Decrease in Aflatoxin M1 Concentration in Milk during Cholesterol Removal by Application of β-Cyclodextrin
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 14
Issue 7
10.3390/toxins14070423
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Characterization of Two Dehydrogenases from Gluconobacter oxydans Involved in the Transformation of Patulin to Ascladiol

by
Edicon T. S. Chan
1,2,
Yan Zhu
2,
Xiu-Zhen Li
2,
Ting Zhou
2,* and
Stephen Y. K. Seah
1,*
1
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
2
Guelph Research and Development Centre, Agriculture and Agriculture-Food Canada, Guelph, ON N1G 5C9, Canada
*
Authors to whom correspondence should be addressed.
Toxins 2022, 14(7), 423; https://doi.org/10.3390/toxins14070423
Submission received: 13 May 2022 / Revised: 3 June 2022 / Accepted: 20 June 2022 / Published: 21 June 2022
(This article belongs to the Special Issue Innovative Mycotoxin Detoxification: Discoveries, Mechanisms and Applications)
Browse Figures
Versions Notes

Abstract

Patulin is a mycotoxin that primarily contaminate apples and apple products. Whole cell or cell-free extracts of Gluconobacter oxydans ATCC 621 were able to transform patulin to E-ascladiol. Proteins from cell-free extracts were separated by anion exchange chromatography and fractions with patulin transformation activity were subjected to peptide mass fingerprinting, enabling the identification of two NADPH dependent short chain dehydrogenases, GOX0525 and GOX1899, with the requisite activity. The genes encoding these enzymes were expressed in E. coli and purified. Kinetic parameters for patulin reduction, as well as pH profiles and thermostability were established to provide further insight on the potential application of these enzymes for patulin detoxification.
Keywords: mycotoxin; patulin; ascladiol; Gluconobacter oxydans; detoxification; enzyme; dehydrogenase mycotoxin; patulin; ascladiol; Gluconobacter oxydans; detoxification; enzyme; dehydrogenase
Graphical Abstract

Share and Cite

MDPI and ACS Style

Chan, E.T.S.; Zhu, Y.; Li, X.-Z.; Zhou, T.; Seah, S.Y.K. Characterization of Two Dehydrogenases from Gluconobacter oxydans Involved in the Transformation of Patulin to Ascladiol. Toxins 2022, 14, 423. https://doi.org/10.3390/toxins14070423

AMA Style

Chan ETS, Zhu Y, Li X-Z, Zhou T, Seah SYK. Characterization of Two Dehydrogenases from Gluconobacter oxydans Involved in the Transformation of Patulin to Ascladiol. Toxins. 2022; 14(7):423. https://doi.org/10.3390/toxins14070423

Chicago/Turabian Style

Chan, Edicon T. S., Yan Zhu, Xiu-Zhen Li, Ting Zhou, and Stephen Y. K. Seah. 2022. "Characterization of Two Dehydrogenases from Gluconobacter oxydans Involved in the Transformation of Patulin to Ascladiol" Toxins 14, no. 7: 423. https://doi.org/10.3390/toxins14070423

APA Style

Chan, E. T. S., Zhu, Y., Li, X.-Z., Zhou, T., & Seah, S. Y. K. (2022). Characterization of Two Dehydrogenases from Gluconobacter oxydans Involved in the Transformation of Patulin to Ascladiol. Toxins, 14(7), 423. https://doi.org/10.3390/toxins14070423

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop