Mycotoxin Production and the Relationship between Microbial Diversity and Mycotoxins in Pyrus bretschneideri Rehd cv. Huangguan Pear
Abstract
:1. Introduction
2. Results
2.1. Microbial Community Composition in Pear Fruits
2.2. Comparison of Microbial Diversity in Pear Fruit
2.3. Correlation Analysis of Fungi and Bacteria
2.4. Metabolic Pathways of Microorganisms
2.5. Mycotoxin Content and Its Relationship with Microbial Composition
3. Discussion
4. Materials and Methods
4.1. Storage Conditions and Sample Preparation of Pear Fruit
4.2. DNA Extraction and Amplicon Sequencing
4.3. Bioinformatic Pipeline for Analysis of Microbial Diversity
4.4. Determination of Mycotoxin Production
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pairs | Bacteria | Fungi | ||||
---|---|---|---|---|---|---|
R2 | P-Adjusted | Sig | R2 | P-Adjusted | Sig | |
BH vs. WH | 0.591978 | 0.011053 | * | 0.321574 | 0.011053 | * |
BH vs. SH | 0.212455 | 0.0465 | * | 0.312754 | 0.011053 | * |
BH vs. XH | 0.721622 | 0.011053 | * | 0.215719 | 0.015714 | * |
BH vs. JH | 0.409482 | 0.011053 | * | 0.532116 | 0.011053 | * |
BH vs. BR | 0.813081 | 0.011053 | * | 0.702342 | 0.011053 | * |
BH vs. WR | 0.81491 | 0.011053 | * | 0.749095 | 0.011053 | * |
BH vs. XR | 0.679522 | 0.011053 | * | 0.747734 | 0.011053 | * |
BH vs. JR | 0.721766 | 0.011053 | * | 0.680768 | 0.011053 | * |
WH vs. SH | 0.396408 | 0.011053 | * | 0.171005 | 0.055739 | |
WH vs. XH | 0.71682 | 0.011053 | * | 0.162669 | 0.070833 | |
WH vs. JH | 0.359256 | 0.011786 | * | 0.348047 | 0.011063 | * |
WH vs. BR | 0.827208 | 0.011053 | * | 0.716855 | 0.011053 | * |
WH vs. WR | 0.658041 | 0.011053 | * | 0.681124 | 0.011053 | * |
WH vs. SR | 0.72838 | 0.011053 | * | 0.688894 | 0.011053 | * |
WH vs. XR | 0.843609 | 0.011053 | * | 0.677885 | 0.011053 | * |
WH vs. JR | 0.859374 | 0.01186 | * | 0.72708 | 0.011053 | * |
SH vs. XH | 0.517982 | 0.011786 | * | 0.164089 | 0.027209 | * |
SH vs. JH | 0.278789 | 0.011053 | * | 0.316119 | 0.011053 | * |
SH vs. BR | 0.651772 | 0.011053 | * | 0.738717 | 0.011053 | * |
SH vs. WR | 0.616787 | 0.011053 | * | 0.695862 | 0.011053 | * |
SH vs. SR | 0.586405 | 0.011053 | * | 0.697443 | 0.011053 | * |
SH vs. XR | 0.586903 | 0.011053 | * | 0.693377 | 0.011053 | * |
SH vs. JR | 0.631411 | 0.011053 | * | 0.735392 | 0.011053 | * |
XH vs. JH | 0.40517 | 0.016193 | * | 0.382327 | 0.011053 | * |
XH vs. BR | 0.887939 | 0.011053 | * | 0.712806 | 0.011053 | * |
XH vs. WR | 0.89198 | 0.011053 | * | 0.688394 | 0.011053 | * |
XH vs. SR | 0.878855 | 0.011053 | * | 0.682479 | 0.011053 | * |
XH vs. XR | 0.89183 | 0.011053 | * | 0.685562 | 0.011053 | * |
XH vs. JR | 0.892796 | 0.011053 | * | 0.715241 | 0.011053 | * |
JH vs. BR | 0.621556 | 0.011053 | * | 0.809491 | 0.011053 | * |
JH vs. WR | 0.551114 | 0.011053 | * | 0.606817 | 0.011053 | * |
JH vs. SR | 0.558436 | 0.011053 | * | 0.761766 | 0.011053 | * |
JH vs. XR | 0.631405 | 0.011053 | * | 0.607294 | 0.011053 | * |
JH vs. JR | 0.638654 | 0.011053 | * | 0.830115 | 0.011053 | * |
BR vs. WR | 0.96884 | 0.011053 | * | 0.894688 | 0.011053 | * |
BR vs. SR | 0.945308 | 0.011538 | * | 0.775024 | 0.011053 | * |
BR vs. XR | 0.975101 | 0.011053 | * | 0.920598 | 0.011053 | * |
BR vs. JR | 0.977461 | 0.011053 | * | 0.83395 | 0.011053 | * |
WR vs. SR | 0.908071 | 0.011786 | * | 0.851352 | 0.011341 | * |
WR vs. XR | 0.983926 | 0.011053 | * | 0.90034 | 0.011053 | * |
WR vs. JR | 0.987103 | 0.011053 | * | 0.958482 | 0.011053 | * |
SR vs. XR | 0.961185 | 0.011053 | * | 0.858435 | 0.011053 | * |
SR vs. JR | 0.955762 | 0.011053 | * | 0.760137 | 0.011063 | * |
XR vs. JR | 0.890881 | 0.011053 | * | 0.961663 | 0.011053 | * |
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Li, H.; Zhang, Y.; Gao, C.; Gao, Q.; Cheng, Y.; Zhao, M.; Guan, J. Mycotoxin Production and the Relationship between Microbial Diversity and Mycotoxins in Pyrus bretschneideri Rehd cv. Huangguan Pear. Toxins 2022, 14, 699. https://doi.org/10.3390/toxins14100699
Li H, Zhang Y, Gao C, Gao Q, Cheng Y, Zhao M, Guan J. Mycotoxin Production and the Relationship between Microbial Diversity and Mycotoxins in Pyrus bretschneideri Rehd cv. Huangguan Pear. Toxins. 2022; 14(10):699. https://doi.org/10.3390/toxins14100699
Chicago/Turabian StyleLi, Huimin, Yang Zhang, Congcong Gao, Qi Gao, Yudou Cheng, Min Zhao, and Junfeng Guan. 2022. "Mycotoxin Production and the Relationship between Microbial Diversity and Mycotoxins in Pyrus bretschneideri Rehd cv. Huangguan Pear" Toxins 14, no. 10: 699. https://doi.org/10.3390/toxins14100699