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Article

Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells

1
Natural Product and Cancer Cell Laboratories, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada
2
Department of Physics and Astronomy, University of Lethbridge, Lethbridge AB T1K 3M4, Canada
3
Department of Earth, Ocean, Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
*
Author to whom correspondence should be addressed.
Toxins 2021, 13(2), 138; https://doi.org/10.3390/toxins13020138
Received: 14 January 2021 / Revised: 7 February 2021 / Accepted: 9 February 2021 / Published: 12 February 2021
(This article belongs to the Special Issue Basic Research for the Potential Use of Plant Toxins)
We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of biological interest. We provide the first report of the effects of extracts prepared from the shrub Symphoricarpos occidentalis in several human cell lines. S. occidentalis (Caprifoliaceae) extracts are cytotoxic, and, strikingly, treated cells undergo light-dependent vacuolation near the nucleus. The range of irradiation is present in standard ambient light and lies in the visible range (400-700 nm). Vacuolization in treated cells can be induced with specific wavelengths of 408 or 660 nm at 1 J/cm2 energies. Vacuolated cells show a striking phenotype of a large perinuclear vacuole (nuclear associated vacuole, NAV) that is distinct from vesicles observed by treatment with an autophagy-inducing agent. Treatment with S. occidentalis extracts and light induces an intense lamin A/C signal at the junction of a nuclear vacuole and the nucleus. Further study of S. occidentalis extracts and vacuolation provide chemical tools that may contribute to the understanding of nuclear envelope organization and human cell biology. View Full-Text
Keywords: Canada; Caprifoliaceae; laminopathies; lamins A/C; nuclear associated vacuole (NAV), nucleus; Pelger-Huët Anomaly; Symphoricarpos occidentalis Canada; Caprifoliaceae; laminopathies; lamins A/C; nuclear associated vacuole (NAV), nucleus; Pelger-Huët Anomaly; Symphoricarpos occidentalis
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MDPI and ACS Style

Tuescher, J.M.; Beck, C.R.; Spencer, L.; Yeremy, B.; Shi, Y.; Andersen, R.J.; Golsteyn, R.M. Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells. Toxins 2021, 13, 138. https://doi.org/10.3390/toxins13020138

AMA Style

Tuescher JM, Beck CR, Spencer L, Yeremy B, Shi Y, Andersen RJ, Golsteyn RM. Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells. Toxins. 2021; 13(2):138. https://doi.org/10.3390/toxins13020138

Chicago/Turabian Style

Tuescher, Jan M.; Beck, Chad R.; Spencer, Locke; Yeremy, Benjamin; Shi, Yutong; Andersen, Raymond J.; Golsteyn, Roy M. 2021. "Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells" Toxins 13, no. 2: 138. https://doi.org/10.3390/toxins13020138

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