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Open AccessArticle

Listeria monocytogenes Interferes with Host Cell Mitosis through Its Virulence Factors InlC and ActA

1
Group of Molecular Microbiology, i3S-Instituto de Investigação e Inovação em Saúde, IBMC, Universidade do Porto, 4200-135 Porto, Portugal
2
Group of Cell Biology of Bacterial Infections, i3S-Instituto de Investigação e Inovação em Saúde, IBMC, Universidade do Porto, 4200-135 Porto, Portugal
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(6), 411; https://doi.org/10.3390/toxins12060411
Received: 15 May 2020 / Revised: 12 June 2020 / Accepted: 15 June 2020 / Published: 20 June 2020
(This article belongs to the Special Issue Toxins and Virulence Factors of Listeria monocytogenes)
Listeria monocytogenes is among the best-characterized intracellular pathogens. Its virulence factors, and the way they interfere with host cells to hijack host functions and promote the establishment and dissemination of the infection, have been the focus of multiple studies over the last 30 years. During cellular infection, L. monocytogenes was shown to induce host DNA damage and delay the host cell cycle to its own benefit. However, whether the cell cycle stage would interfere with the capacity of Listeria to infect human cultured cell lines was never assessed. We found here that L. monocytogenes preferentially infects cultured cells in G2/M phases. Inside G2/M cells, the bacteria lead to an increase in the overall mitosis duration by delaying the mitotic exit. We showed that L. monocytogenes infection causes a sustained activation of the spindle assembly checkpoint, which we correlated with the increase in the percentage of misaligned chromosomes detected in infected cells. Moreover, we demonstrated that chromosome misalignment in Listeria-infected cells required the function of two Listeria virulence factors, ActA and InlC. Our findings show the pleiotropic role of Listeria virulence factors and their cooperative action in successfully establishing the cellular infection. View Full-Text
Keywords: Listeria monocytogenes; cellular infection; virulence factors; mitosis; cell cycle progression Listeria monocytogenes; cellular infection; virulence factors; mitosis; cell cycle progression
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Costa, A.C.; Pinheiro, J.; Reis, S.A.; Cabanes, D.; Sousa, S. Listeria monocytogenes Interferes with Host Cell Mitosis through Its Virulence Factors InlC and ActA. Toxins 2020, 12, 411.

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