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Article

Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers

1
Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA
2
Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA
3
USDA-ARS, Forage-Animal Production Research Unit, Lexington, KY 40546, USA
4
Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, KY 40546, USA
5
Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA
*
Author to whom correspondence should be addressed.
Current Address: Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NM 88003, USA.
Current Address: Department of Agriculture, Texas A&M University-Commerce, Commerce, TX 75428, USA.
Toxins 2020, 12(12), 744; https://doi.org/10.3390/toxins12120744
Received: 4 November 2020 / Revised: 24 November 2020 / Accepted: 24 November 2020 / Published: 26 November 2020
(This article belongs to the Special Issue Global Impact of Ergot Alkaloids)
Fescue toxicosis impacts beef cattle production via reductions in weight gain and muscle development. Isoflavone supplementation has displayed potential for mitigating these effects. The objective of the current study was to evaluate isoflavone supplementation with fescue seed consumption on rumen and serum metabolomes. Angus steers (n = 36) were allocated randomly in a 2 × 2 factorial arrangement of treatments including endophyte-infected (E+) or endophyte-free (E−) tall fescue seed, with (P+) or without (P−) isoflavones. Steers were provided a basal diet with fescue seed for 21 days, while isoflavones were orally administered daily. Following the trial, blood and rumen fluid were collected for metabolite analysis. Metabolites were extracted and then analyzed by UPLC-MS. The MAVEN program was implemented to identify metabolites for MetaboAnalyst 4.0 and SAS 9.4 statistical analysis. Seven differentially abundant metabolites were identified in serum by isoflavone treatment, and eleven metabolites in the rumen due to seed type (p < 0.05). Pathways affected by treatments were related to amino acid and nucleic acid metabolism in both rumen fluid and serum (p < 0.05). Therefore, metabolism was altered by fescue seed in the rumen; however, isoflavones altered metabolism systemically to potentially mitigate detrimental effects of seed and improve animal performance. View Full-Text
Keywords: beef cattle; endophyte; ergot alkaloid; fescue toxicosis; isoflavone; metabolites beef cattle; endophyte; ergot alkaloid; fescue toxicosis; isoflavone; metabolites
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MDPI and ACS Style

Ault-Seay, T.B.; Melchior-Tiffany, E.A.; Clemmons, B.A.; Cordero, J.F.; Bates, G.E.; Flythe, M.D.; Klotz, J.L.; Ji, H.; Goodman, J.P.; McLean, K.J.; Myer, P.R. Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers. Toxins 2020, 12, 744. https://doi.org/10.3390/toxins12120744

AMA Style

Ault-Seay TB, Melchior-Tiffany EA, Clemmons BA, Cordero JF, Bates GE, Flythe MD, Klotz JL, Ji H, Goodman JP, McLean KJ, Myer PR. Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers. Toxins. 2020; 12(12):744. https://doi.org/10.3390/toxins12120744

Chicago/Turabian Style

Ault-Seay, Taylor B.; Melchior-Tiffany, Emily A.; Clemmons, Brooke A.; Cordero, Juan F.; Bates, Gary E.; Flythe, Michael D.; Klotz, James L.; Ji, Huihua; Goodman, Jack P.; McLean, Kyle J.; Myer, Phillip R. 2020. "Rumen and Serum Metabolomes in Response to Endophyte-Infected Tall Fescue Seed and Isoflavone Supplementation in Beef Steers" Toxins 12, no. 12: 744. https://doi.org/10.3390/toxins12120744

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