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Communication

Laser Ablation Remote-Electrospray Ionisation Mass Spectrometry (LARESI MSI) Imaging—New Method for Detection and Spatial Localization of Metabolites and Mycotoxins Produced by Moulds

1
Department of Environmental Biotechnology, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
2
Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave., 35-959 Rzeszów, Poland
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(11), 720; https://doi.org/10.3390/toxins12110720
Received: 30 October 2020 / Revised: 13 November 2020 / Accepted: 15 November 2020 / Published: 18 November 2020
(This article belongs to the Special Issue Occurrence and Risk Assessment of Mycotoxins)
To date, no method has been developed to assess the distribution of mycotoxins on the surface of grains, or other plant material, and the depth of their penetration into the interior. The Infrared (IR) Laser Ablation-Remote-Electrospray Ionization (LARESI) platform coupled to a tandem mass spectrometer (MS/MS), measuring in selected reaction monitoring (SRM) mode, was employed for the targeted imaging of selected metabolites of Aspergillus fumigatus, including mycotoxins in biological objects for the first time. This methodology allowed for the localisation of grain metabolites and fungal metabolites of grain infected by this mould. The distribution of metabolites in spelt grain was differentiated: fumigaclavine C, fumitremorgin C, and fumiquinazoline D were located mainly in the embryo, brevianamide F in the seed coat, and fumagillin in the endosperm. The LARESI mass spectrometry imaging method can be used in the future for the metabolomic analysis of mould metabolites in various plants and agricultural products. View Full-Text
Keywords: mass spectrometry imagining; laser ablation; desorption/ionization mass spectrometry metabolites; metabolomic analysis; mycotoxins mass spectrometry imagining; laser ablation; desorption/ionization mass spectrometry metabolites; metabolomic analysis; mycotoxins
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MDPI and ACS Style

Szulc, J.; Ruman, T. Laser Ablation Remote-Electrospray Ionisation Mass Spectrometry (LARESI MSI) Imaging—New Method for Detection and Spatial Localization of Metabolites and Mycotoxins Produced by Moulds. Toxins 2020, 12, 720. https://doi.org/10.3390/toxins12110720

AMA Style

Szulc J, Ruman T. Laser Ablation Remote-Electrospray Ionisation Mass Spectrometry (LARESI MSI) Imaging—New Method for Detection and Spatial Localization of Metabolites and Mycotoxins Produced by Moulds. Toxins. 2020; 12(11):720. https://doi.org/10.3390/toxins12110720

Chicago/Turabian Style

Szulc, Justyna, and Tomasz Ruman. 2020. "Laser Ablation Remote-Electrospray Ionisation Mass Spectrometry (LARESI MSI) Imaging—New Method for Detection and Spatial Localization of Metabolites and Mycotoxins Produced by Moulds" Toxins 12, no. 11: 720. https://doi.org/10.3390/toxins12110720

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