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Aberrant Expressional Profiling of Known MicroRNAs in the Liver of Silver Carp (Hypophthalmichthys molitrix) Following Microcystin-LR Exposure Based on samllRNA Sequencing

College of Life Science, Henan Normal University, Xinxiang 453007, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2020, 12(1), 41; https://doi.org/10.3390/toxins12010041
Received: 6 December 2019 / Revised: 2 January 2020 / Accepted: 7 January 2020 / Published: 9 January 2020
Microcystin-LR (MC-LR) poses a serious threat to human health due to its hepatotoxicity. However, the specific molecular mechanism of miRNAs in MC-LR-induced liver injury has not been determined. The aim of the present study was to determine whether miRNAs are regulated in MC-LR-induced liver toxicity by using high-throughput sequencing. Our research demonstrated that 53 miRNAs and 319 miRNAs were significantly changed after 24 h of treatment with MC-LR (50 and 200 μg/kg, respectively) compared with the control group. GO enrichment analysis revealed that these target genes were related to cellular, metabolic, and single-organism processes. Furthermore, KEGG pathway analysis demonstrated that the target genes of differentially expressed miRNAs in fish liver were primarily involved in the insulin signaling pathway, PPAR signaling pathway, Wnt signaling pathway, and transcriptional misregulation in cancer. Moreover, we hypothesized that 4 miRNAs (miR-16, miR-181a-3p, miR-451, and miR-223) might also participate in MC-LR-induced toxicity in multiple organs of the fish and play regulatory roles according to the qPCR analysis results. Taken together, our results may help to elucidate the biological function of miRNAs in MC-LR-induced toxicity. View Full-Text
Keywords: Microcystin-LR; miRNA; Illumina sequencing; silver carp; hepatotoxicity Microcystin-LR; miRNA; Illumina sequencing; silver carp; hepatotoxicity
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Feng, Y.; Chen, X.; Ma, J.; Zhang, B.; Li, X. Aberrant Expressional Profiling of Known MicroRNAs in the Liver of Silver Carp (Hypophthalmichthys molitrix) Following Microcystin-LR Exposure Based on samllRNA Sequencing. Toxins 2020, 12, 41.

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